TNFSF8 Polyclonal antibody

TNFSF8 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 17852-1-AP

Synonyms

CD153, CD30 L, CD30 ligand, CD30L, CD30LG, TNFSF8

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Validation Data Gallery

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  WB analysis of MCF-7 using 17852-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 17852-1-AP (TNFSF8 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 17852-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17852-1-AP (TNFSF8 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 17852-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17852-1-AP (TNFSF8 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 17852-1-AP is TNFSF8 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TNFSF8 fusion protein Ag12303
• Full Name: tumor necrosis factor (ligand) superfamily, member 8
• Calculated Molecular Weight: 234 aa, 26 kDa
• Observed Molecular Weight: 35-40 kDa
• GenBank Accession Number: BC093630
• Gene Symbol: TNFSF8
• Gene ID (NCBI): 944
• RRID: AB_2878452
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TNFSF8, also named as CD30LG and CD153, is a member of the tumor necrosis factor (TNF) receptor superfamily, is a surface antigen used as a clinical marker for Hodgkin lymphoma and related hematologic malignancies. It induces proliferation of T-cells. CD30L enhanced the proliferation of CD3-activated T cells, but induced differential responses, including cell death, in several CD30-positive lymphoma-derived cell lines. For Glycosylation, the MW in WB detection is about 35-40 kDa.