CD30/TNFRSF8 Polyclonal antibody

CD30/TNFRSF8 Polyclonal Antibody for IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 55132-1-AP

Synonyms

CD30, Ki-1 antigen, Ki 1 antigen, Ki 1, D1S166E

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Validation Data Gallery

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  IHC staining of human lymphoma using 55132-1-AP

  Immunohistochemical analysis of paraffin-embedded human T-cell anaplastic large cell lymphoma (ALCL) tissue slide using 55132-1-AP (CD30 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 55132-1-AP

  Immunohistochemical analysis of paraffin-embedded human T-cell anaplastic large cell lymphoma (ALCL) tissue slide using 55132-1-AP (CD30 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 55132-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 55132-1-AP (CD30 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 55132-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 55132-1-AP (CD30 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human lymphoma using 55132-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human T-cell anaplastic large cell lymphoma (ALCL) tissue using CD30 antibody (55132-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human lymphoma using 55132-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human T-cell anaplastic large cell lymphoma (ALCL) tissue using CD30 antibody (55132-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive IHC detected in: human lymphoma tissue, human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human lymphoma tissue

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

55132-1-AP targets CD30/TNFRSF8 in IHC, IF-P, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: tumor necrosis factor receptor superfamily, member 8
• Calculated Molecular Weight: 64 kDa
• GenBank Accession Number: NM_001243
• Gene Symbol: CD30
• Gene ID (NCBI): 943
• RRID: AB_2881274
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: 0.1M NaHCO3, 0.1M glycine, 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD30 is a transmembrane glycoprotein belonging to the tumor necrosis factor receptor superfamily. CD30 expression is found on activated T and B cells. CD30 is useful in diagnosis of classic Hodgkin's lymphoma, anaplastic large cell lymphoma and embryonal carcinoma.