CoraLite®594-conjugated TNF Alpha Monoclonal antibody
TNF Alpha Monoclonal Antibody for IF
Host / Isotype
Mouse / IgG2b
Reactivity
human
Applications
IF-P
Conjugate
CoraLite®594 Fluorescent Dye
CloneNo.
7B8A11
Cat no : CL594-60291
Synonyms
Validation Data Gallery
Tested Applications
Positive IF-P detected in | human breast cancer tissue |
Recommended dilution
Application | Dilution |
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Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
CL594-60291 targets TNF Alpha in IF-P applications and shows reactivity with human samples.
Tested Reactivity | human |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Immunogen | TNF Alpha fusion protein Ag11413 |
Full Name | tumor necrosis factor (TNF superfamily, member 2) |
Calculated Molecular Weight | 233 aa, 26 kDa |
GenBank Accession Number | BC028148 |
Gene Symbol | TNF alpha |
Gene ID (NCBI) | 7124 |
RRID | AB_2883456 |
Conjugate | CoraLite®594 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 588 nm / 604 nm |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TNF, as also known as TNF-alpha, or cachectin, is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. It is expressed as a 26 kDa membrane bound protein and is then cleaved by TNF-alpha converting enzyme (TACE) to release the soluble 17 kDa monomer, which forms homotrimers in circulation. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. The antibody is conjugated with CL594, Ex/Em 593 nm/614 nm.
Protocols
Product Specific Protocols | |
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IF protocol for CL594 TNF Alpha antibody CL594-60291 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |