CoraLite® Plus 488-conjugated TNF-alpha Monoclonal antibody

• Featured Product
• KD/KO Validated

TNF-alpha Monoclonal Antibody for IF-P

Host / Isotype

Mouse / IgG2b

Reactivity

human and More (1)

Applications

WB, IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Publications

3

CloneNo.

7B8A11

Cat no : CL488-60291

Synonyms

TNF, TNF alpha, TNF-a, Intracellular domain 2, Intracellular domain 1

---

Validation Data Gallery

• 

  IF Staining of human breast cancer using CL488-60291

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CL488-60291 (TNF alpha antibody) at dilution of 1:50.

• 

  IF Staining of human breast cancer using CL488-60291

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CL488-60291 (TNF alpha antibody) at dilution of 1:50.

---

Tested Applications

• Positive IF-P detected in: human breast cancer tissue

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 2 publications below

Product Information

CL488-60291 targets TNF-alpha in WB, IF-P applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: TNF-alpha fusion protein Ag11413
• Full Name: tumor necrosis factor (TNF superfamily, member 2)
• Calculated Molecular Weight: 233 aa, 26 kDa
• GenBank Accession Number: BC028148
• Gene Symbol: TNF-alpha
• Gene ID (NCBI): 7124
• RRID: AB_2883136
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

TNF, as also known as TNF-alpha, or cachectin, is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. It is expressed as a 26 kDa membrane bound protein and is then cleaved by TNF-alpha converting enzyme (TACE) to release the soluble 17 kDa monomer, which forms homotrimers in circulation. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, INS resistance, and cancer. The antibody is conjugated with CL488, Ex/Em 488 nm/515 nm.

Publications

Species	Application	Title
human	IF	PLoS Pathog; SARS-CoV-2 spike-induced syncytia are senescent and contribute to exacerbated heart failure; Authors - Huilong Li
	WB	Biomed Pharmacother; Nr2e1 ablation impairs liver glucolipid metabolism and induces inflammation, high-fat diets amplify the damage.; Authors - Qing Xiong
mouse	IF	Biomacromolecules; Multifunctional Polymer Vesicles for Synergistic Antibiotic-Antioxidant Treatment of Bacterial Keratitis; Authors - Qiumeng Chen