- Featured Product
- KD/KO Validated
TNF Alpha Monoclonal antibody, PBS Only
TNF Alpha Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
human, rat
Applications
WB, IHC, IF-P, Indirect ELISA
Conjugate
Unconjugated
CloneNo.
7B8A11
Cat no : 60291-1-PBS
Synonyms
Validation Data Gallery
Product Information
60291-1-PBS targets TNF Alpha in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, rat samples.
Tested Reactivity | human, rat |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Immunogen | TNF Alpha fusion protein Ag11413 |
Full Name | tumor necrosis factor (TNF superfamily, member 2) |
Calculated Molecular Weight | 233 aa, 26 kDa |
Observed Molecular Weight | 17-22 kDa |
GenBank Accession Number | BC028148 |
Gene Symbol | TNF alpha |
Gene ID (NCBI) | 7124 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS Only |
Storage Conditions | Store at -80°C. |
Background Information
TNF, as also known as TNF-alpha, or cachectin, is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. It is expressed as a 26 kDa membrane bound protein and is then cleaved by TNF-alpha converting enzyme (TACE) to release the soluble 17 kDa monomer, which forms homotrimers in circulation. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, INS resistance, and cancer.