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  • KD/KO Validated

ASC/TMS1 Polyclonal antibody

ASC/TMS1 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (3)

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 10500-1-AP

Synonyms

PYCARD, hASC, Caspase recruitment domain-containing protein 5, CARD5, ASC



Tested Applications

Positive WB detected inHL-60 cells, A549 cells, Raji cells, U-937 cells, THP-1 cells
Positive IP detected inHL-60 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10500-1-AP targets ASC/TMS1 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, pig, canine, bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ASC/TMS1 fusion protein Ag0774
Full Name PYD and CARD domain containing
Calculated Molecular Weight 25 kDa
Observed Molecular Weight 22-25 kDa
GenBank Accession NumberBC004470
Gene Symbol ASC/TMS1
Gene ID (NCBI) 29108
RRIDAB_2174862
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PYCARD , also known as ASC or TMS1, is a 195 amino acid protein containing an N-terminal Pyrin-like domain (PYD) and an 87 residue C-terminal Caspase-associated recruitment domain (CARD). It promotes caspase-mediated apoptosis which is mediated predominantly through the activation of caspase-9.

Protocols

Product Specific Protocols
WB protocol for ASC/TMS1 antibody 10500-1-APDownload protocol
IHC protocol for ASC/TMS1 antibody 10500-1-APDownload protocol
IF protocol for ASC/TMS1 antibody 10500-1-APDownload protocol
IP protocol for ASC/TMS1 antibody 10500-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Research (Wash D C)

Herpetrione, a New Type of PPARα Ligand as a Therapeutic Strategy Against Nonalcoholic Steatohepatitis

Authors - Lang Linghu
WB

Transl Res

IMM-H004 therapy for permanent focal ischemic cerebral injury via CKLF1/CCR4-mediated NLRP3 inflammasome activation.

Authors - Q D Ai
humanWB,IHC

Cancer Res

Silencing of TMS1/ASC promotes resistance to anoikis in breast epithelial cells.

Authors - Parsons Melissa J MJ
  • KD Validated
humanWB

Cancer Res

Role of hMOF-dependent histone H4 lysine 16 acetylation in the maintenance of TMS1/ASC gene activity.

Authors - Kapoor-Vazirani Priya P
WB,IF,IP

Cell Mol Life Sci

circACTA2 inhibits NLRP3 inflammasome-mediated inflammation via interacting with NF-κB in vascular smooth muscle cells

Authors - Yang Bai
WB

Vaccines (Basel)

Jatrorrhizine Suppresses Murine-Norovirus-Triggered N-GSDMD-Dependent Pyroptosis in RAW264.7 Macrophages

Authors - Ming Fu