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- KD/KO Validated
ASC/TMS1 Polyclonal antibody
ASC/TMS1 Polyclonal Antibody for WB, IHC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (3)
Applications
WB, IHC, IF, IP, CoIP, ELISA
Conjugate
Unconjugated
236
Cat no : 10500-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HL-60 cells, A549 cells, Raji cells, U-937 cells, THP-1 cells |
Positive IP detected in | HL-60 cells |
Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 4 publications below |
WB | See 206 publications below |
IHC | See 21 publications below |
IF | See 47 publications below |
IP | See 5 publications below |
ELISA | See 1 publications below |
CoIP | See 4 publications below |
Product Information
10500-1-AP targets ASC/TMS1 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Cited Reactivity | human, pig, canine, bovine |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | ASC/TMS1 fusion protein Ag0774 |
Full Name | PYD and CARD domain containing |
Calculated Molecular Weight | 25 kDa |
Observed Molecular Weight | 22-25 kDa |
GenBank Accession Number | BC004470 |
Gene Symbol | ASC/TMS1 |
Gene ID (NCBI) | 29108 |
RRID | AB_2174862 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PYCARD , also known as ASC or TMS1, is a 195 amino acid protein containing an N-terminal Pyrin-like domain (PYD) and an 87 residue C-terminal Caspase-associated recruitment domain (CARD). It promotes caspase-mediated apoptosis which is mediated predominantly through the activation of caspase-9.
Protocols
Product Specific Protocols | |
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WB protocol for ASC/TMS1 antibody 10500-1-AP | Download protocol |
IHC protocol for ASC/TMS1 antibody 10500-1-AP | Download protocol |
IF protocol for ASC/TMS1 antibody 10500-1-AP | Download protocol |
IP protocol for ASC/TMS1 antibody 10500-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Research (Wash D C) Herpetrione, a New Type of PPARα Ligand as a Therapeutic Strategy Against Nonalcoholic Steatohepatitis | ||
Transl Res IMM-H004 therapy for permanent focal ischemic cerebral injury via CKLF1/CCR4-mediated NLRP3 inflammasome activation. | ||
Cancer Res Silencing of TMS1/ASC promotes resistance to anoikis in breast epithelial cells.
| ||
Cancer Res Role of hMOF-dependent histone H4 lysine 16 acetylation in the maintenance of TMS1/ASC gene activity. | ||
Cell Mol Life Sci circACTA2 inhibits NLRP3 inflammasome-mediated inflammation via interacting with NF-κB in vascular smooth muscle cells | ||
Vaccines (Basel) Jatrorrhizine Suppresses Murine-Norovirus-Triggered N-GSDMD-Dependent Pyroptosis in RAW264.7 Macrophages |