TMPRSS2 Polyclonal antibody

• 

  WB analysis using 14437-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14437-1-AP (TMPRSS2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 14437-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14437-1-AP (TMPRSS2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 14437-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 14437-1-AP (TMPRSS2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 14437-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 14437-1-AP (TMPRSS2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 14437-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14437-1-AP (TMPRSS2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 14437-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14437-1-AP (TMPRSS2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 14437-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14437-1-AP (TMPRSS2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 14437-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14437-1-AP (TMPRSS2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 14437-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14437-1-AP (TMPRSS2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 14437-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14437-1-AP (TMPRSS2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human tonsillitis using 14437-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using TMPRSS2 antibody (14437-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human tonsillitis using 14437-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using TMPRSS2 antibody (14437-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

TMPRSS2 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Cat No. 14437-1-AP

Publications(33)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF-P, ELISA

Synonyms

Transmembrane protease serine 2 non-catalytic chain, Transmembrane protease serine 2 catalytic chain, PRSS10, PP9284, EC:3.4.21.122

---

Tested Applications

• Positive WB detected in: COLO 320 cells, Caco-2 cells, T-47D cells
• Positive IHC detected in: human colon cancer tissue, human prostate cancer tissue, mouse kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human tonsillitis tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 24 publications below
• IHC: See 4 publications below
• IF: See 9 publications below

Product Information

14437-1-AP targets TMPRSS2 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, monkey
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TMPRSS2 fusion protein Ag5824
• Full Name: transmembrane protease, serine 2
• Calculated Molecular Weight: 54 kDa
• Observed Molecular Weight: 70, 54, 31 kDa
• GenBank Accession Number: BC051839
• Gene Symbol: TMPRSS2
• Gene ID (NCBI): 7113
• RRID: AB_2878058
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O15393
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TMPRSS2, also named as PRSS10, is a type II transmembrane serine protease which is highly expressed by the epithelium of the human prostate gland. TMPRSS2 may contribute to prostate tumour metastasis via the activation of PAR-2. TMPRSS2 is a Serine protease that proteolytically cleaves and activates the viral spike glycoproteins which facilitate virus-cell membrane fusions. TMPRSS2 is a host cell factor that is critical for the spread of several clinically relevant viruses, including influenza A viruses and coronaviruses (PMID: 23468491, 30626688). SARS-CoV-2 uses the SARS-CoV receptor ACE2 for entry and the serine protease TMPRSS2 for S protein priming. The initial spike protein priming by TMPRSS2 is essential for the entry and viral spread of SARS-CoV-2 through interaction with the ACE2 receptor (PMID: 32142651, 30626688 ). Camostat mesylate, an inhibitor of TMPRSS2, can block SARS-CoV-2 infection of lung cells (PMID: 32142651). The MW of TMPRSS2 is about 65-70 kDa. It can be cleaved into some chains with MW 54 kDa, 31 kDa and 26 kDa (PMID: 25734995, 20382709, 26018085).

Publications

Species	Application	Title
human	WB	Nat Microbiol; SARS-CoV-2 Omicron is an immune escape variant with an altered cell entry pathway.; Authors - Brian J Willett
human	WB	Allergy; Distinct expression of SARS-CoV-2 receptor ACE2 correlates with endotypes of chronic rhinosinusitis with nasal polyps.; Authors - Ming Wang
human	WB	Eur Respir J; Influenza virus infection increases ACE2 expression and shedding in human small airway epithelial cells.; Authors - Kelly S Schweitzer
human	WB	Theranostics; Environmentally-induced mdig contributes to the severity of COVID-19 through fostering expression of SARS-CoV-2 receptor NRPs and glycan metabolism.; Authors - Qian Zhang
human	WB	Mol Ther Nucleic Acids; The accessible promoter-mediated supplementary effect of host factors provides new insight into the tropism of SARS-CoV-2.; Authors - Guifang Du
human	IF	PLoS Pathog; Infection of human Nasal Epithelial Cells with SARS-CoV-2 and a 382-nt deletion isolate lacking ORF8 reveals similar viral kinetics and host transcriptional profiles.; Authors - Akshamal M Gamage

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Christine (Verified Customer) (12-14-2021)

I transfected HEK293T cells with a plasmid encoding the long isoform of TMPRSS2 with a FLAG tag at the C terminus. I analysed the expression of TMPRSS2 (not expressed endogenously by HEK293T) with the TMPRSS2 antibody but did not detect any difference between transfected and untransfected cells, whereas I could clearly detect it with an anti FLAG antibody. So this antibody might work better on endogenous TMPRSS2 and not very well against my tagged version?

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: transfected HEK293T