TMPPE Recombinant antibody

TMPPE Uni-rAb^TM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

241180C8

Cat no : 84028-2-RR

Synonyms

241180C8

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Validation Data Gallery

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  WB analysis using 84028-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84028-2-RR (TMPPE antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 84028-2-RR

  Biolayer interferometry (BLl) kinetic assays of 84028-2-RR against Human TMPPE were performed. The affinity constant is 82.2 pM.

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Tested Applications

• Positive WB detected in: HepG2 cells, HeLa cells, HEK-293T cells, U-251 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84028-2-RR is TMPPE Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: TMPPE fusion protein Ag19556
• Full Name: transmembrane protein with metallophosphoesterase domain
• Calculated Molecular Weight: 453 aa, 49 kDa
• Observed Molecular Weight: 45-50 kDa
• GenBank Accession Number: BC136873
• Gene Symbol: TMPPE
• Gene ID (NCBI): 643853
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.