TMEM50B Polyclonal antibody, PBS Only

TMEM50B Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 15941-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of mouse testis using 15941-1-AP (same clone as 15941-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 15941-1-AP (TMEM50B antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15941-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse heart using 15941-1-AP (same clone as 15941-1-PBS)

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 15941-1-AP (TMEM50B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15941-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse testis using 15941-1-AP (same clone as 15941-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 15941-1-AP (TMEM50B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15941-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 15941-1-AP (same clone as 15941-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15941-1-AP (TMEM50B antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15941-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 15941-1-AP (same clone as 15941-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15941-1-AP (TMEM50B antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15941-1-PBS in a different storage buffer formulation.

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Product Information

15941-1-PBS targets TMEM50B in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TMEM50B fusion protein Ag8220
• Full Name: transmembrane protein 50B
• Calculated Molecular Weight: 15 kDa
• Observed Molecular Weight: 14 kDa, 30 kDa, 50 kDa
• GenBank Accession Number: BC000569
• Gene Symbol: TMEM50B
• Gene ID (NCBI): 757
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

TMEM50B, originally referred to as C21orf4, is a predicted transmembrane protein, estimated to contain four transmembrane-spanning domains with both the carboxy- and amino-terminals being cytoplasmic (PMID: 10077530; 18541381). Human TMEM50B shares sequence homologies of around 90-100% with mouse and rat Tmem50b. In a Down syndrome mouse model, Ts1Cje, Tmem50b is shown to be one of the few genes significantly over-expressed during cerebellar development (PMID: 15590701). It has been reported that rodent Tmem50b is a developmentally-regulated intracellular ER and Golgi apparatus membrane protein that may prove important for correct brain development through functions associated with precursor cells and glia (PMID: 18541381). TMEM50B can exist as a dimer and a trimer conformation (PMID: 18541381).