TMEM39A Polyclonal antibody

TMEM39A Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 20430-1-AP

Synonyms

SUSR2, TMEM39A, transmembrane protein 39A

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Validation Data Gallery

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  WB analysis using 20430-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20430-1-AP (TMEM39A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 20430-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20430-1-AP (TMEM39A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of U2OS using 20430-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using TMEM39A antibody (20430-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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  IF Staining of U-251 using 20430-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U-251 cells using TMEM39A antibody (20430-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Tested Applications

• Positive WB detected in: U-87 MG cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: U2OS cells, U-251 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

20430-1-AP targets TMEM39A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TMEM39A fusion protein Ag14263
• Full Name: transmembrane protein 39A
• Calculated Molecular Weight: 488 aa, 56 kDa
• Observed Molecular Weight: 48-56 kDa
• GenBank Accession Number: BC021277
• Gene Symbol: TMEM39A
• Gene ID (NCBI): 55254
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Transmembrane protein 39A (TMEM39A, also called SUSR2) encoding an ER-localized transmembrane protein, is a susceptibility locus for multiple autoimmune diseases (PMID: 31806350).