TMEM35 Polyclonal antibody

TMEM35 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 24786-1-AP

Synonyms

TMEM35, transmembrane protein 35

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Validation Data Gallery

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  WB analysis of mouse cerebellum using 24786-1-AP

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 24786-1-AP (TMEM35 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 24786-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 24786-1-AP (TMEM35 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse cerebellum tissue, mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 1 publications below

Product Information

24786-1-AP targets TMEM35 in WB, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TMEM35 fusion protein Ag20589
• Full Name: transmembrane protein 35
• Calculated Molecular Weight: 167 aa, 18 kDa
• Observed Molecular Weight: 20 kDa
• GenBank Accession Number: BC078658
• Gene Symbol: TMEM35
• Gene ID (NCBI): 59353
• RRID: AB_2879723
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB,IF	Am J Respir Cell Mol Biol; Nicotine-induced ER Stress and ASM Cell Proliferation is Mediated by α7nAChR and Chaperones-RIC-3 and TMEM35; Authors - Niyati A Borkar
		bioRxiv; Mechanism of NACHO-mediated assembly of pentameric ligand-gated ion channels; Authors - Yogesh Hooda