TMEM195 Polyclonal antibody

TMEM195 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 21355-1-AP

Synonyms

Alkylglycerol monooxygenase, TMEM195, transmembrane protein 195

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Validation Data Gallery

• 

  WB analysis of HepG2 using 21355-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 21355-1-AP (TMEM195 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 21355-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 21355-1-AP (TMEM195 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 21355-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 21355-1-AP (TMEM195 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 21355-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 21355-1-AP (TMEM195 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 21355-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 21355-1-AP (TMEM195 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver using 21355-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 21355-1-AP (TMEM195 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human liver cancer using 21355-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21355-1-AP (TMEM195 antibody) at dilution of 1:200 (under 10x lens).

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Tested Applications

• Positive WB detected in: HepG2 cells, L02 cells, MCF-7 cells, mouse liver tissue
• Positive IHC detected in: human liver tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

The immunogen of 21355-1-AP is TMEM195 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TMEM195 fusion protein Ag15774
• Full Name: transmembrane protein 195
• Calculated Molecular Weight: 445 aa, 52 kDa
• Observed Molecular Weight: 52 kDa
• GenBank Accession Number: BC108676
• Gene Symbol: TMEM195
• Gene ID (NCBI): 392636
• RRID: AB_2878845
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	J Infect Dis; Biochemical Characterization of AGMO Variants Implicated in Relapses in Visceral Leishmaniasis.; Authors - Katrin Watschinger
human	WB	Hum Genet; Biallelic variants in AGMO with diminished enzyme activity are associated with a neurodevelopmental disorder.; Authors - Volkan Okur