Validation Data Gallery
|Positive WB detected in||HSC-T6 cells, THP-1 cells, HEK-293 cells, HepG2 cells, Jurkat cells|
|Positive IHC detected in||human tonsillitis tissue, human spleen tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:2000-1:10000|
|Immunohistochemistry (IHC)||IHC : 1:500-1:2000|
|Sample-dependent, check data in validation data gallery|
66680-1-Ig targets TMEM173/STING in WB, IHC, IF applications and shows reactivity with Human, Rat samples.
|Tested Reactivity||Human, Rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||TMEM173/STING fusion protein Ag20363|
|Full Name||transmembrane protein 173|
|Calculated molecular weight||379 aa, 42 kDa|
|Observed molecular weight||37 kDa|
|GenBank accession number||BC047779|
|Gene ID (NCBI)||340061|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Stimulator of interferon genes (STING, also known as ERIS, MITA and MPYS, and encoded by TMEM173) is a transmembrane adaptor protein that facilitates innate immune signalling (PMID: 18724357). STING is widely expressed in various cell types such as endothelial cell, epithelial cells, T cells, macrophages and dendritic cells (PMID: 26603901). It is predominantly located in endoplasmic reticulum (ER). STING functions as a sensor of cytosolic DNA and promotes the production of type I interferons and and pro-inflammatory cytokines. STING is a 379 amino acid protein with a calculated molecular wight of 42 kDa. It has been observed at 35-40 kDa (PMID: 27324217; 29632140; 30918080), and 70-80 kDa corresponding to the expected size of a STING dimer (PMID: 25790474; 29491158).
Staphylococcus aureus Induces IFN-β Production via a CARMA3-Independent Mechanism.
STING agonist and IDO inhibitor combination therapy inhibits tumor progression in murine models of colorectal cancer.
Lipotoxicity-induced STING1 activation stimulates MTORC1 and restricts hepatic lipophagy.
Cell Death Differ
The DJ1-Nrf2-STING axis mediates the neuroprotective effects of Withaferin A in Parkinson's disease.
Golgi apparatus-synthesized sulfated glycosaminoglycans mediate polymerization and activation of the cGAMP sensor STING.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Will (Verified Customer) (04-25-2019)
good antibody, will order again