TMEM173/STING Monoclonal antibody

• Featured Product
• KD/KO Validated

TMEM173/STING Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Rat and More (2)

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

31

CloneNo.

1F1E1

Reviews (1)

Cat no : 66680-1-Ig

Synonyms

FLJ38577, hMITA, hSTING, Mediator of IRF3 activation, MITA, MPYS, NET23, STING, TMEM173, TMEM173/STING, transmembrane protein 173

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Validation Data Gallery

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  WB analysis using 66680-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66680-1-Ig (TMEM173/STING antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control.

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  WB analysis of THP-1 using 66680-1-Ig

  WB result of TMEM173/STING antibody (66680-1-Ig; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-TMEM173/STING transfected THP-1 cells.

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  WB analysis of HSC-T6 using 66680-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66680-1-Ig (TMEM173 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 66680-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66680-1-Ig (TMEM173 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 66680-1-Ig

  WB result of TMEM173/STING antibody (66680-1-Ig; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-TMEM173/STING transfected HEK-293 cells.

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  IHC staining of human tonsillitis using 66680-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66680-1-Ig (TMEM173/STING antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 66680-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66680-1-Ig (TMEM173/STING antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human spleen using 66680-1-Ig

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 66680-1-Ig (TMEM173/STING antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human spleen using 66680-1-Ig

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 66680-1-Ig (TMEM173/STING antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, HSC-T6 cells, HEK-293 cells, THP-1 cells, MOLT-4 cells, Jurkat cells
• Positive IHC detected in: human tonsillitis tissue, human spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 6 publications below
• WB: See 22 publications below
• IHC: See 3 publications below
• IF: See 15 publications below
• IP: See 2 publications below

Product Information

66680-1-Ig targets TMEM173/STING in WB, IHC, IF, IP, ELISA applications and shows reactivity with Human, Rat samples.

• Tested Reactivity: Human, Rat
• Cited Reactivity: human, rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: TMEM173/STING fusion protein Ag20363
• Full Name: transmembrane protein 173
• Calculated Molecular Weight: 379 aa, 42 kDa
• Observed Molecular Weight: 37 kDa
• GenBank Accession Number: BC047779
• Gene Symbol: STING
• Gene ID (NCBI): 340061
• RRID: AB_2882034
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Stimulator of interferon genes (STING, also known as ERIS, MITA and MPYS, and encoded by TMEM173) is a transmembrane adaptor protein that facilitates innate immune signaling (PMID: 18724357). STING is widely expressed in various cell types such as endothelial cells, epithelial cells, T cells, macrophages, and dendritic cells (PMID: 26603901). It is predominantly located in the endoplasmic reticulum (ER). STING functions as a sensor of cytosolic DNA and promotes the production of type I interferons and pro-inflammatory cytokines. STING is a 379 amino acid protein with a calculated molecular weight of 42 kDa. It has been observed at 35-40 kDa (PMID: 27324217; 29632140; 30918080), and 70-80 kDa corresponding to the expected size of a STING dimer (PMID: 25790474; 29491158).

Publications

Species	Application	Title
human	WB, IF	Immunity; Golgi apparatus-synthesized sulfated glycosaminoglycans mediate polymerization and activation of the cGAMP sensor STING.; Authors - Run Fang
mouse	WB	Cell Death Differ; The DJ1-Nrf2-STING axis mediates the neuroprotective effects of Withaferin A in Parkinson's disease.; Authors - Miao Zhao ; KO Validated
mouse	WB	Cell Death Differ; Inhibition of cGAS-STING by JQ1 alleviates oxidative stress-induced retina inflammation and degeneration.; Authors - Ming Zou
human,mouse	IF	Autophagy; Lipotoxicity-induced STING1 activation stimulates MTORC1 and restricts hepatic lipophagy.; Authors - Kunpeng Liu ; KO Validated
human	IF	Oncogene; GBP3 promotes glioblastoma resistance to temozolomide by enhancing DNA damage repair.; Authors - Hui Xu
mouse	IF	Free Radic Biol Med; STING promotes ferroptosis through NCOA4-dependent ferritinophagy in acute kidney injury; Authors - Lini Jin

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Will (Verified Customer) (04-25-2019)

good antibody, will order again

• Applications: Western Blot,