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  • KD/KO Validated

TMEM173/STING Monoclonal antibody

TMEM173/STING Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Rat and More (2)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1F1E1

Cat no : 66680-1-Ig

Synonyms

FLJ38577, hMITA, hSTING, Mediator of IRF3 activation, MITA, MPYS, NET23, STING, TMEM173, TMEM173/STING, transmembrane protein 173



Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, HSC-T6 cells, HEK-293 cells, THP-1 cells, MOLT-4 cells, Jurkat cells
Positive IHC detected inhuman tonsillitis tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Product Information

66680-1-Ig targets TMEM173/STING in WB, IP, IHC, IF, ELISA applications and shows reactivity with Human, Rat samples.

Tested Reactivity Human, Rat
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen TMEM173/STING fusion protein Ag20363
Full Name transmembrane protein 173
Calculated molecular weight 379 aa, 42 kDa
Observed molecular weight 37 kDa
GenBank accession numberBC047779
Gene symbol TMEM173
Gene ID (NCBI) 340061
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Stimulator of interferon genes (STING, also known as ERIS, MITA and MPYS, and encoded by TMEM173) is a transmembrane adaptor protein that facilitates innate immune signaling (PMID: 18724357). STING is widely expressed in various cell types such as endothelial cells, epithelial cells, T cells, macrophages, and dendritic cells (PMID: 26603901). It is predominantly located in the endoplasmic reticulum (ER). STING functions as a sensor of cytosolic DNA and promotes the production of type I interferons and pro-inflammatory cytokines. STING is a 379 amino acid protein with a calculated molecular weight of 42 kDa. It has been observed at 35-40 kDa (PMID: 27324217; 29632140; 30918080), and 70-80 kDa corresponding to the expected size of a STING dimer (PMID: 25790474; 29491158).

Protocols

Product Specific Protocols
WB protocol for TMEM173/STING antibody 66680-1-IgDownload protocol
IHC protocol for TMEM173/STING antibody 66680-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Oxid Med Cell Longev

Hydrogen Sulfide Ameliorated High Choline-Induced Cardiac Dysfunction by Inhibiting cGAS-STING-NLRP3 Inflammasome Pathway

Authors - Lu Bai
humanWB

Pathogens

Staphylococcus aureus Induces IFN-β Production via a CARMA3-Independent Mechanism.

Authors - Yang Zhou
mouseWB

Cell Immunol

STING agonist and IDO inhibitor combination therapy inhibits tumor progression in murine models of colorectal cancer.

Authors - Jiaqi Shi
humanWB,IF

Cancer Cell Int

AMICA1 is a diagnostic and prognostic biomarker and induces immune cells infiltration by activating cGAS-STING signaling in lung adenocarcinoma.

Authors - Ziyang Feng
humanWB

Front Pharmacol

Curcumol Suppresses CCF-Mediated Hepatocyte Senescence Through Blocking LC3B-Lamin B1 Interaction in Alcoholic Fatty Liver Disease.

Authors - Xiaoyu Qi
humanIP

J Innate Immun

Human STING Is Regulated by an Autoinhibitory Mechanism for Type I Interferon Production.

Authors - Shishi Qi
  • KO Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Will (Verified Customer) (04-25-2019)

good antibody, will order again

  • Applications: Western Blot,