Validation Data Gallery
|Positive WB detected in||HepG2 cells, L02 cells, pig liver tissue, mouse lung tissue, mouse pancreas tissue, mouse brain tissue, mouse liver tissue, rat liver tissue|
|Positive IP detected in||mouse liver tissue|
|Positive IHC detected in||human liver tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells, HeLa cells|
|Western Blot (WB)||WB : 1:1000-1:8000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
The immunogen of 21620-1-AP is TMED9 Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse, rat, pig|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||TMED9 fusion protein Ag13813|
|Full Name||transmembrane emp24 protein transport domain containing 9|
|Calculated molecular weight||235 aa, 27 kDa|
|Observed molecular weight||24-27 kDa|
|GenBank accession number||BC001123|
|Gene ID (NCBI)||54732|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
TMED9 (also named as GMP25, GP25L2 or p25) is a member of the EMP24/GP25L family. TMED9 probably forms hetero-oligomeric complexes with TMED7 (p27), TMED2 (p24), and TMED10 (p23) (PMID: 10359607). Located in the endoplasmic reticulum and the Golgi, TMED9 appears to be involved in vesicular protein trafficking, mainly in the early secretory pathway (PMID: 9472029; 10359607).
|Product Specific Protocols|
|WB protocol for TMED9 antibody 21620-1-AP||Download protocol|
|IHC protocol for TMED9 antibody 21620-1-AP||Download protocol|
|IF protocol for TMED9 antibody 21620-1-AP||Download protocol|
|IP protocol for TMED9 antibody 21620-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Small Molecule Targets TMED9 and Promotes Lysosomal Degradation to Reverse Proteinopathy.
A Translocation Pathway for Vesicle-Mediated Unconventional Protein Secretion.
A new type of ERGIC-ERES membrane contact mediated by TMED9 and SEC12 is required for autophagosome biogenesis.
ACBD3 modulates KDEL receptor interaction with PKA for its trafficking via tubulovesicular carrier.
High expression of transmembrane P24 trafficking protein 9 predicts poor prognosis in breast carcinoma.
Adv Sci (Weinh)
TMED3 Complex Mediates ER Stress-Associated Secretion of CFTR, Pendrin, and SARS-CoV-2 Spike.