TMED4 Polyclonal antibody

• Featured Product
• KD/KO Validated

TMED4 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 14141-1-AP

Synonyms

Putative NF-kappa-B-activating protein 156, p24alpha3, p24 family protein alpha-3, GMP25iso, ERS25

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Validation Data Gallery

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  WB analysis of mouse liver using 14141-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 14141-1-AP (TMED4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 14141-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 14141-1-AP (TMED4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 14141-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14141-1-AP (TMED4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 14141-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 14141-1-AP (TMED4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 14141-1-AP

  IP result of anti-TMED4 (IP:14141-1-AP, 4ug; Detection:14141-1-AP 1:700) with mouse liver tissue lysate 6000 ug.

• 

  IF Staining of HepG2 using 14141-1-AP

  Immunofluorescent analysis of HepG2 cells using 14141-1-AP (TMED4 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse liver tissue, HepG2 cells
• Positive IP detected in: mouse liver tissue
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 2 publications below

Product Information

14141-1-AP targets TMED4 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TMED4 fusion protein Ag5234
• Full Name: transmembrane emp24 protein transport domain containing 4
• Calculated Molecular Weight: 227 aa, 26 kDa
• Observed Molecular Weight: 26 kDa
• GenBank Accession Number: BC057851
• Gene Symbol: TMED4
• Gene ID (NCBI): 222068
• RRID: AB_10642696
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Cell Res; A new type of ERGIC-ERES membrane contact mediated by TMED9 and SEC12 is required for autophagosome biogenesis.; Authors - Shulin Li ; KD Validated
human	WB	Ann Transl Med; Screening of the novel immune-suppressive biomarkers of TMED family and whether knockdown of TMED2/3/4/9 inhibits cell migration and invasion in breast cancer; Authors - Zheng Fang ; KD Validated