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  • Tested Applications
  • Product Information
  • Publications (5)
  • Protocols
  • Featured Product
  • KD/KO Validated

TM9SF4 Polyclonal antibody

TM9SF4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25595-1-AP

Synonyms

KIAA0255, TM9SF4



Tested Applications

Positive WB detected inA375 cells, A431 cells, HepG2 cells
Positive IHC detected inmouse colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25595-1-AP targets TM9SF4 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TM9SF4 fusion protein Ag22335
Full Name transmembrane 9 superfamily protein member 4
Calculated Molecular Weight 642 aa, 75 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC021107
Gene Symbol TM9SF4
Gene ID (NCBI) 9777
RRIDAB_2880149
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for TM9SF4 antibody 25595-1-APDownload protocol
IHC protocol for TM9SF4 antibody 25595-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

  • Journal Impact Factor
  • Most recent
SpeciesApplicationTitle
humanWB,IHC

Oncogene

Knockdown of TM9SF4 boosts ER stress to trigger cell death of chemoresistant breast cancer cells.

Authors - Yifei Zhu
  • KD Validated
mouseWB,IF

Stem Cell Res Ther

TM9SF4 is a novel regulator in lineage commitment of bone marrow mesenchymal stem cells to either osteoblasts or adipocytes

Authors - Libo Yu
  • KO Validated
humanIHC

J Exp Clin Cancer Res

CircCEMIP promotes anoikis-resistance by enhancing protective autophagy in prostate cancer cells.

Authors - Ying Yu
mouse,humanWB,IHC,IF

Cell Mol Gastroenterol Hepatol

TM9SF4 Is a Crucial Regulator of Inflammation and ER Stress in Inflammatory Bowel Disease

Authors - Mingxu Xie
  • KD Validated
human,mouseWB,IHC

Nat Commun

TM9SF4 is an F-actin disassembly factor that promotes tumor progression and metastasis

Authors - Zhaoyue Meng
  • KD Validated