TLE3 Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

TLE3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 22094-1-PBS

Synonyms

HsT18976, GRG3, GRG 3, ESG3, ESG

---

Validation Data Gallery

• 

  WB analysis of HEK-293 using 22094-1-AP (same clone as 22094-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 22094-1-AP (TLE3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22094-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 22094-1-AP (same clone as 22094-1-PBS)

  WB result of TLE3 antibody (22094-1-AP, 1:4000) with si-Control and si-TLE3 transfected HEK293 cells. This data was developed using the same antibody clone with 22094-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A431 using 22094-1-AP (same clone as 22094-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 22094-1-AP (TLE3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22094-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 22094-1-AP (same clone as 22094-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 22094-1-AP (TLE3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22094-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 22094-1-AP (same clone as 22094-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 22094-1-AP (TLE3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22094-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HepG2 using 22094-1-AP (same clone as 22094-1-PBS)

  IP result of anti-TLE3 (IP:22094-1-AP, 4ug; Detection:22094-1-AP 1:1000) with HepG2 cells lysate 2400ug. This data was developed using the same antibody clone with 22094-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 22094-1-AP (same clone as 22094-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22094-1-AP (TLE3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22094-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 22094-1-AP (same clone as 22094-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22094-1-AP (TLE3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22094-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 22094-1-AP (same clone as 22094-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22094-1-AP (TLE3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22094-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 22094-1-AP (same clone as 22094-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22094-1-AP (TLE3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22094-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 22094-1-AP (same clone as 22094-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TLE3 antibody (22094-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 22094-1-PBS in a different storage buffer formulation.

---

Product Information

22094-1-PBS targets TLE3 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TLE3 fusion protein Ag17300
• Full Name: transducin-like enhancer of split 3 (E(sp1) homolog, Drosophila)
• Calculated Molecular Weight: 772 aa, 83 kDa
• Observed Molecular Weight: 90 kDa
• GenBank Accession Number: BC015729
• Gene Symbol: TLE3
• Gene ID (NCBI): 7090
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Transducin-like enhancer of split 3(TLE3) is a corepressor that binds to a variant of transciption factor, which mediated by CTNNB1 and TCF family members in Wnt signaling. It's suggested that effect of full-length TLE family members be regulated by association with dominant-negative AES.