TLE3 Polyclonal antibody

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  WB analysis using 11372-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11372-1-AP (TLE3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 11372-1-AP

  WB result of TLE3 antibody (11372-1-AP, 1:500) with si-Control and si-TLE3 transfected HEK293 cells.

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  IP experiment of HepG2 using 11372-1-AP

  IP result of anti-TLE3 (IP:11372-1-AP, 4ug; Detection:11372-1-AP 1:2000) with HepG2 cells lysate 1080 ug.

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  IHC staining of human breast cancer using 11372-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11372-1-AP (TLE3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 11372-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11372-1-AP (TLE3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 11372-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TLE3 antibody (11372-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

• Featured Product
• KD/KO Validated

TLE3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 11372-1-AP

Publications(23)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA

Synonyms

Enhancer of split groucho-like protein 3, ESG, ESG3, GRG 3, GRG3

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 6 publications below
• WB: See 19 publications below
• IHC: See 3 publications below
• IF: See 5 publications below
• CoIP: See 2 publications below
• ChIP: See 7 publications below

Product Information

11372-1-AP targets TLE3 in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag1930

  Product name: Recombinant human TLE3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-350 aa of BC015729

  Sequence: MYPQGRHPAPHQPGQPGFKFTVAESCDRIKDEFQFLQAQYHSLKVEYDKLANEKTEMQRHYVMYYEMSYGLNIEMHKQTEIAKRLNTILAQIMPFLSQEHQQQVAQAVERAKQVTMTELNAIIGQQQLQAQHLSHATHGPPVQLPPHPSGLQPPGIPPVTGSSSGLLALGALGSQAHLTVKDEKNHHELDHRERESSANNSVSPSESLRASEKHRGSADYSMEAKKRKAEEKDSLSRYDSDGDKSDDLVVDVSNEDPATPRVSPAHSPPENGLDKARSLKKDAPTSPASVASSSSTPSSKTKDLGHNDKSSTPGLKSNTPTPRNDAPTPGTSTTPGLRSMPGKPPGMDPI

• Full Name: transducin-like enhancer of split 3 (E(sp1) homolog, Drosophila)
• Calculated Molecular Weight: 772 aa, 83 kDa
• Observed Molecular Weight: 83 kDa
• GenBank Accession Number: BC015729
• Gene Symbol: TLE3
• Gene ID (NCBI): 7090
• RRID: AB_2203743
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q04726
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TLE3, also named as KIAA1547 and ESG3, belongs to the WD repeat Groucho/TLE family. It is a transcriptional corepressor that binds to a number of transcription factors. It inhibits the transcriptional activation mediated by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. TLE3 is a member of the transducin-like enhancer of split (TLE) family of proteins that have been implicated in the tumorgenesis and classification of sarcomas. It is a candidate biomarker of response to taxane

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,CoIP,ChIP	Nature; Distinct structural classes of activating FOXA1 alterations in advanced prostate cancer.; Authors - Abhijit Parolia ; KD Validated
mouse	WB	Cell Stem Cell; Lats1/2 Sustain Intestinal Stem Cells and Wnt Activation through TEAD-Dependent and Independent Transcription.; Authors - Qi Li
mouse	WB,ChIP	Cell Metab; TLE3 is a dual-function transcriptional coregulator of adipogenesis.; Authors - Villanueva Claudio J CJ
mouse	WB	Cell Metab; Adipose subtype-selective recruitment of TLE3 or Prdm16 by PPARγ specifies lipid storage versus thermogenic gene programs.; Authors - Villanueva Claudio J CJ ; KO Validated
human	WB,chIP	J Clin Invest; Small molecule JQ1 promotes prostate cancer invasion via BET-independent inactivation of FOXA1.; Authors - Leiming Wang
mouse	chIP	J Exp Med; Tle corepressors are differentially partitioned to instruct CD8+ T cell lineage choice and identity.; Authors - Shaojun Xing ; KO Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Tongbin (Verified Customer) (08-05-2020)

I did western blots using mouse heart samples. I observed a TLE3 band with correct molecular weight of about 80kD.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse heart tissue