TIMM17B Polyclonal antibody

TIMM17B Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Publications

9

Cat no : 11062-1-AP

Synonyms

DXS9822, JM3, TIM17B, TIMM17B

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Validation Data Gallery

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  WB analysis of mouse heart using 11062-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 11062-1-AP (TIMM17B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 11062-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 11062-1-AP (TIMM17B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of PC-3 using 11062-1-AP

  IP result of anti-TIMM17B (IP:11062-1-AP, 4ug; Detection:11062-1-AP 1:1000) with PC-3 cells lysate 1575 ug.

• 

  IHC staining of human colon cancer using 11062-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11062-1-AP (TIMM17B antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

• Positive WB detected in: mouse heart tissue, mouse liver tissue
• Positive IP detected in: PC-3 cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 7 publications below

Product Information

11062-1-AP targets TIMM17B in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TIMM17B fusion protein Ag1504
• Full Name: translocase of inner mitochondrial membrane 17 homolog B (yeast)
• Calculated Molecular Weight: 18 kDa
• Observed Molecular Weight: 17-20 kDa
• GenBank Accession Number: BC010142
• Gene Symbol: TIMM17B
• Gene ID (NCBI): 10245
• RRID: AB_2201995
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Cell Metab; Acylglycerol Kinase Maintains Metabolic State and Immune Responses of CD8+ T Cells.; Authors - Zhilin Hu
human	WB	Mol Cell; Acylglycerol Kinase Mutated in Sengers Syndrome Is a Subunit of the TIM22 Protein Translocase in Mitochondria.; Authors - Milena Vukotic
human	WB	Mol Cell; Sengers Syndrome-Associated Mitochondrial Acylglycerol Kinase Is a Subunit of the Human TIM22 Protein Import Complex.; Authors - Yilin Kang
human		Cell Rep; TIM23 facilitates PINK1 activation by safeguarding against OMA1-mediated degradation in damaged mitochondria; Authors - Shiori Akabane
		Sci Rep; The presence of disulfide bonds reveals an evolutionarily conserved mechanism involved in mitochondrial protein translocase assembly.; Authors - Lidia Wrobel
human	WB	Infect Immun; The type III system-secreted effector EspZ localizes to host mitochondria and interacts with the translocase of inner mitochondrial membrane 17b.; Authors - Shames Stephanie R SR