TIMM17A Polyclonal antibody

• Featured Product
• KD/KO Validated

TIMM17A Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 11189-1-AP

Synonyms

MIMT17, TIM17, TIM17A, TIMM17, TIMM17A

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Validation Data Gallery

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  WB analysis using 11189-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11189-1-AP (TIMM17A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 11189-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11189-1-AP (TIMM17A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 11189-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11189-1-AP (TIMM17A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells, K-562 cells, MCF-7 cells, Neuro-2a cells, NIH/3T3 cells, U-251 cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 2 publications below

Product Information

11189-1-AP targets TIMM17A in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TIMM17A fusion protein Ag1665
• Full Name: translocase of inner mitochondrial membrane 17 homolog A (yeast)
• Calculated Molecular Weight: 18 kDa
• Observed Molecular Weight: 18 kDa
• GenBank Accession Number: BC015098
• Gene Symbol: TIMM17A
• Gene ID (NCBI): 10440
• RRID: AB_2271661
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human		Cell Rep; TIM23 facilitates PINK1 activation by safeguarding against OMA1-mediated degradation in damaged mitochondria; Authors - Shiori Akabane
human	WB	EMBO Rep; UBXN1 maintains ER proteostasis and represses UPR activation by modulating translation; Authors - Brittany A Ahlstedt
		J Mol Cell Cardiol; Embryonic cardiomyocytes can orchestrate various cell protective mechanisms to survive mitochondrial stress.; Authors - Manuela Magarin
human	WB,IHC	Proteomics; Quantitative proteomics study of breast cancer cell lines isolated from a single patient: discovery of TIMM17A as a marker for breast cancer.; Authors - Xu Xiaoen X ; KD Validated
rat	WB,IHC	Int J Mol Med; 'Governor vessel-unblocking and mind-regulating' acupuncture therapy ameliorates cognitive dysfunction in a rat model of middle cerebral artery occlusion.; Authors - Xuan Su
human	WB	J Cell Biol; ROMO1 is a constituent of the human presequence translocase required for YME1L protease import.; Authors - Frank Richter