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TH Monoclonal antibody

TH Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2H7B7

Cat no : 66334-1-Ig

Synonyms

TH, TYH, Tyrosine 3 hydroxylase, Tyrosine 3 monooxygenase, tyrosine hydroxylase



Tested Applications

Positive WB detected inPC-12 cells, SH-SY5Y cells
Positive IF/ICC detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:3000-1:20000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66334-1-Ig targets TH in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TH fusion protein Ag23075
Full Name tyrosine hydroxylase
Calculated Molecular Weight 528 aa, 59 kDa
Observed Molecular Weight 55 kDa
GenBank Accession NumberBC104967
Gene Symbol TH
Gene ID (NCBI) 7054
RRIDAB_2881714
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TH(Tyrosine 3-monooxygenase) converts L-tyrosine to L-3,4-dihydroxyphenylalanine (L-DOPA), the essential and rate-limiting step to formation of DA and other catecholamines. TH plays an important role in the physiology of adrenergic neurons and can be used as a marker for DA and noradrenergic neurons.

Protocols

Product Specific Protocols
WB protocol for TH antibody 66334-1-IgDownload protocol
IF protocol for TH antibody 66334-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB,IF

J Ginseng Res

The necessity of eliminating the interference of panaxatriol saponins to maximize the preventive effect of panaxadiol saponins against Parkinson's disease in rats

Authors - Yanwei Wang
humanWB

Mol Oncol

Norepinephrine-CREB1-miR-373 axis promotes progression of colon cancer.

Authors - Jia Han
WB

Front Cell Dev Biol

RAB39B Deficiency Impairs Learning and Memory Partially Through Compromising Autophagy.

Authors - Mengxi Niu
humanIF

Antioxidants (Basel)

Mitochondrial Calcium-Triggered Oxidative Stress and Developmental Defects in Dopaminergic Neurons Differentiated from Deciduous Teeth-Derived Dental Pulp Stem Cells with MFF Insufficiency

Authors - Xiao Sun
WB,IF

Bone Joint Res

High-intensity interval training improves fatty infiltration in the rotator cuff through the β3 adrenergic receptor in mice

Authors - Hecheng Zhou
IHC

Biomed Pharmacother

Nardosinone regulates the slc38a2 gene to alleviate Parkinson's symptoms in rats through the GABAergic synaptic and cAMP pathways.

Authors - Li-Hua Bian