TGDS Polyclonal antibody

TGDS Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17310-1-AP

Synonyms

dTDP D glucose 4,6 dehydratase, SDR2E1, TDP glucose 4,6 dehydratase, TDPGD, TGDS

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Validation Data Gallery

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  WB analysis of human kidney using 17310-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 17310-1-AP (TGDS antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 17310-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 17310-1-AP (TGDS antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human bladder using 17310-1-AP

  Immunohistochemical analysis of paraffin-embedded human bladder using 17310-1-AP (TGDS antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human bladder using 17310-1-AP

  Immunohistochemical analysis of paraffin-embedded human bladder using 17310-1-AP (TGDS antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung cancer using 17310-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 17310-1-AP (TGDS antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung cancer using 17310-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 17310-1-AP (TGDS antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: human kidney tissue, mouse kidney tissue
• Positive IHC detected in: human bladder tissue, human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 17310-1-AP is TGDS Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TGDS fusion protein Ag11276
• Full Name: TDP-glucose 4,6-dehydratase
• Calculated Molecular Weight: 350 aa, 40 kDa
• Observed Molecular Weight: 45-48 kDa
• GenBank Accession Number: BC033675
• Gene Symbol: TGDS
• Gene ID (NCBI): 23483
• RRID: AB_10694428
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.