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TFAM Polyclonal antibody

TFAM Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IP, IHC, IF, chIP, ELISA

Conjugate

Unconjugated

Cat no : 22586-1-AP

Synonyms

Cell and organelle markers, Mitochondria Marker, Mitochondrial Marker, Mitochondrion Marker, MtTF1, mtTFA, TCF 6, TCF6, TCF6L1, TCF6L2, TCF6L3, TFAM, Transcription factor 6, Transcription factor 6 like 2



Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells, MCF-7 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

22586-1-AP targets TFAM in WB, IP, IHC, IF, chIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, chicken, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TFAM fusion protein Ag18299
Full Name transcription factor A, mitochondrial
Calculated molecular weight 246 aa, 29 kDa
Observed molecular weight 25 kDa
GenBank accession numberBC126366
Gene symbol TFAM
Gene ID (NCBI) 7019
RRIDAB_11182588
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TFAM, also named as TCF6L2, MtTF1, TCF6, TCF6L1, TCF6L3 and mtTFA, is a mitochondrial transcription factor that is a key activator of mitochondrial transcription as well as a participant in mitochondrial genome replication. It is involved in mitochondrial transcription regulation. TFAM is required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. It activates transcription by binding immediately upstream of transcriptional start sites. TFAM is able to unwind and bend DNA.

Protocols

Product Specific Protocols
WB protocol for TFAM antibody 22586-1-APDownload protocol
IHC protocol for TFAM antibody 22586-1-APDownload protocol
IF protocol for TFAM antibody 22586-1-APDownload protocol
IP protocol for TFAM antibody 22586-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Nature

A plant-derived natural photosynthetic system for improving cell anabolism

Authors - Pengfei Chen
humanWB

Cell Res

Mitochondria-localized cGAS suppresses ferroptosis to promote cancer progression

Authors - Shiqiao Qiu
IHC

Adv Mater

Neonatal Tissue-derived Extracellular Vesicle Therapy (NEXT): A Potent Strategy for Precision Regenerative Medicine

Authors - Peng Lou
WB,IHC

Circulation

Extracellular Tuning of Mitochondrial Respiration Leads to Aortic Aneurysm.

Authors - Jorge Oller
IHC

Cell Metab

Mitochondrial Damage and Activation of the STING Pathway Lead to Renal Inflammation and Fibrosis.

Authors - Ki Wung Chung
humanWB

Nat Cell Biol

Mitochondrial RNA granules are fluid condensates positioned by membrane dynamics.

Authors - Timo Rey

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Sebastian (Verified Customer) (10-25-2023)

Good detection signals for TFAM in western blot of liver from old and young mice, some unspecific bands were slightly visible

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2500
  • Cell Tissue Type: liver
FH

Tanusree (Verified Customer) (12-18-2019)

Product worked well in WB at 1:500 dilution.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Mouse brain