Transferrin/TF Polyclonal antibody

• Featured Product
• KD/KO Validated

Transferrin/TF Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

59

Reviews (1)

Cat no : 17435-1-AP

Synonyms

TF, transferrin, Siderophilin, Serotransferrin, PRO2086

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Validation Data Gallery

• 

  WB analysis using 17435-1-AP

  human urine lysates were subjected to SDS PAGE followed by western blot with 17435-1-AP (Transferrin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat kidney using 17435-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 17435-1-AP (Transferrin antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat kidney using 17435-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 17435-1-AP (Transferrin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 17435-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 17435-1-AP (Transferrin antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 17435-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 17435-1-AP (Transferrin antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 17435-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 17435-1-AP (Transferrin antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human hepatocirrhosis using 17435-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 17435-1-AP (Transferrin antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human hepatocirrhosis using 17435-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 17435-1-AP (Transferrin antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human colon using 17435-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 17435-1-AP (Transferrin antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 17435-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17435-1-AP (Transferrin antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human placenta using 17435-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17435-1-AP (Transferrin antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 17435-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17435-1-AP (Transferrin antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 17435-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17435-1-AP (Transferrin antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 17435-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 17435-1-AP (Transferrin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: human urine sample, mouse kidney tissue, mouse spleen tissue, rat kidney tissue
• Positive IHC detected in: human hepatocirrhosis tissue, human placenta tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 52 publications below
• IHC: See 9 publications below
• IF: See 2 publications below
• IP: See 2 publications below

Product Information

17435-1-AP targets Transferrin/TF in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, monkey, duck
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Transferrin/TF fusion protein Ag11504
• Full Name: transferrin
• Calculated Molecular Weight: 698 aa, 77 kDa
• Observed Molecular Weight: 77 kDa
• GenBank Accession Number: BC059367
• Gene Symbol: TF
• Gene ID (NCBI): 7018
• RRID: AB_2035023
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

What is the tissue specificity of TF?

Transferrin is synthesized mainly in the liver.

What is the calculated molecular weight of TF?

77 kDa 

How many iron atoms can bind to TF?

Each of the two lobes of TF has one high-affinity Fe(III) binding site. Since binding of iron and bicarbonate 

releases free H+ ions, affinity decreases in acidic conditions, in which TF also remains bound to its receptor.

What is the primary role of TF?

TF serves as a serum metal-binding protein, playing a key role in iron metabolism and delivery for erythropoiesis.

What is the role of TF in human diseases?

TF deficiency results in an exhibition of congenital atransferrinemia (hypotransferrinemia) in which there is a 

decrease in hepcidin expression, causing increased iron levels. 

Is TF essential for iron delivery?

Congenital atransferrinemia results in an iron overload in some tissues, such as the liver, suggesting that while  TF is not essential for iron delivery to all organs, it is needed for iron level regulation.

In what circumstances do TF levels decrease?

Inflammation and iron overload both can cause a decline in TF levels.

How does TF affect hormonal balance?

TF positively regulates expression of hepcidin, a peptide hormone synthesized by the liver. 

How does TF deficiency cause increased iron levels? 

TF regulates hepcidin, which in turn is a negative regulator of intestinal iron absorption, resulting in TF 

deficiency causing both anemia, and, paradoxically, increased iron levels in some tissues. 

PMIDs: 23046645, 9635730, 22294463, 24589273

Publications

Species	Application	Title
human	WB	Nature; 7-Dehydrocholesterol dictates ferroptosis sensitivity; Authors - Yaxu Li
human	WB	Mol Cell; Maintaining Iron Homeostasis Is the Key Role of Lysosomal Acidity for Cell Proliferation.; Authors - Ross A Weber
human,mouse	WB	Sci Adv; The adipocyte-enriched secretory protein tetranectin exacerbates type 2 diabetes by inhibiting insulin secretion from β cells; Authors - Fen Liu
mouse	WB	Research (Wash D C); ATP5O Hypo-crotonylation Caused by HDAC2 Hyper-Phosphorylation Is a Primary Detrimental Factor for Downregulated Phospholipid Metabolism under Chronic Stress; Authors - Liang-Jian Chen
mouse	WB	Biomaterials; The protein corona hampers the transcytosis of transferrin-modified nanoparticles through blood-brain barrier and attenuates their targeting ability to brain tumor.; Authors - Wei Xiao
mouse	WB	Haematologica; Gastrointestinal iron excretion and reversal of iron excess in a mouse model of inherited iron excess.; Authors - Courtney J Mercadante ; KO Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Aditya (Verified Customer) (11-01-2024)

great with icc

• Applications: Immunohistochemistry