TEX12 Polyclonal antibody

TEX12 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IF, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 17068-1-AP

Synonyms

testis expressed 12, TEX12

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Validation Data Gallery

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  IHC staining of human testis using 17068-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17068-1-AP (TEX12 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 17068-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17068-1-AP (TEX12 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 17068-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17068-1-AP (TEX12 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 17068-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17068-1-AP (TEX12 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 17068-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17068-1-AP (TEX12 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 17068-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17068-1-AP (TEX12 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive IHC detected in: human testis tissue, mouse testis tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 2 publications below

Product Information

17068-1-AP targets TEX12 in IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TEX12 fusion protein Ag10893
• Full Name: testis expressed 12
• Calculated Molecular Weight: 123 aa, 14 kDa
• Observed Molecular Weight: 14 kDa
• GenBank Accession Number: BC029506
• Gene Symbol: TEX12
• Gene ID (NCBI): 56158
• RRID: AB_2201875
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	IF	Sci Adv; The histone modification reader ZCWPW1 is required for meiosis prophase I in male but not in female mice.; Authors - Miao Li
mouse	IF	Reproduction; New allele of mouse DNA/RNA helicase senataxin causes meiotic arrest and infertility; Authors - Yasuhiro Fujiwara
		Adv Sci (Weinh); HSF5 Deficiency Causes Male Infertility Involving Spermatogenic Arrest at Meiotic Prophase I in Humans and Mice; Authors - Mohan Liu
		Cell Death Differ; Deficiency of cancer/testis antigen gene CT55 causes male infertility in humans and mice; Authors - Guohui Zhang