Tie2 Polyclonal antibody

Tie2 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

20

Cat no : 19157-1-AP

Synonyms

TEK, hTIE2, Endothelial tyrosine kinase, EC:2.7.10.1, CD202B

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Validation Data Gallery

• 

  WB analysis of mouse lung using 19157-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 19157-1-AP (Tie2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 19157-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 19157-1-AP (Tie2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse lung using 19157-1-AP

  IP result of anti-Tie2 (IP:19157-1-AP, 4ug; Detection:19157-1-AP 1:500) with mouse lung tissue lysate 4000ug.

• 

  IHC staining of human placenta using 19157-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 19157-1-AP (Tie2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 19157-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 19157-1-AP (Tie2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 19157-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 19157-1-AP (Tie2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse lung tissue, mouse liver tissue
• Positive IP detected in: mouse lung tissue
• Positive IHC detected in: human placenta tissue, mouse kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 15 publications below
• IHC: See 3 publications below
• IF: See 3 publications below

Product Information

19157-1-AP targets Tie2 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Tie2 fusion protein Ag13523
• Full Name: TEK tyrosine kinase, endothelial
• Calculated Molecular Weight: 1124 aa, 126 kDa
• Observed Molecular Weight: 140 kDa
• GenBank Accession Number: BC035514
• Gene Symbol: Tie2
• Gene ID (NCBI): 7010
• RRID: AB_10644297
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Tie2 (also known as TEK) is a tyrosine-protein kinase expressed almost exclusively on endothelial cells. It contains two immunoglobulin-like domains, three epidermal growth factor (EGF)--like domains and three fibronectin type III repeats. Tie2 acts as a cell-surface receptor for ANGPT1, ANGPT2, and ANGPT4 and regulates angiogenesis, endothelial cell survival, proliferation, migration, adhesion and cell spreading, reorganization of the actin cytoskeleton, but also maintenance of vascular quiescence. Mutations in the gene Tie2 are associated with inherited venous malformations of the skin and mucous membranes. Human Tie2 has a calculated molecular weight of 126 kDa. As a result of glycosylation, the apparent molecular mass of Tie2 is approximately 140-160 kDa.

Publications

Species	Application	Title
human	IHC	Ther Adv Med Oncol; Development and external validation of a composite immune-clinical prognostic model associated with EGFR mutation in East-Asian patients with lung adenocarcinoma.; Authors - Chengming Liu
human	IHC	Mol Cancer Ther; JQ1 induces DNA damage and apoptosis, and inhibits tumor growth in a patient-derived xenograft model of cholangiocarcinoma.; Authors - Patrick L Garcia
human	IF	Front Cell Dev Biol; Inhibiting Heat Shock Protein 90 Protects Nucleus Pulposus-Derived Stem/Progenitor Cells From Compression-Induced Necroptosis and Apoptosis.; Authors - Binwu Hu
human	WB	Stem Cell Res Ther; Human endometrium-derived stem cell improves cardiac function after myocardial ischemic injury by enhancing angiogenesis and myocardial metabolism.; Authors - Xuemei Fan
rat	WB	Stem Cell Res Ther; MicroRNA-9 modified bone marrow-derived mesenchymal stem cells (BMSCs) repair severe acute pancreatitis (SAP) via inducing angiogenesis in rats.; Authors - Daohai Qian
human	WB	J Mol Cell Cardiol; BMP9 attenuates occurrence of venous malformation by maintaining endothelial quiescence and strengthening vessel walls via SMAD1/5/ID1/α-SMA pathway.; Authors - Yongyun Li

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