TDRKH specific Polyclonal antibody

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  WB analysis of NCCIT using 13528-1-AP

  NCCIT cell were subjected to SDS PAGE followed by western blot with 13528-1-AP (TDRKH specific antibody at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 13528-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13528-1-AP (TDRKH specific antibody at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 13528-1-AP

  IP result of anti-TDRKH specific (IP:13528-1-AP, 4ug; Detection:13528-1-AP 1:7000) with mouse brain tissue lysate 4000ug.

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  IHC staining of human gliomas using 13528-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13528-1-AP (TDRKH specific antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 13528-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13528-1-AP (TDRKH specific antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 13528-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 13528-1-AP (TDRKH specific antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human gliomas using 13528-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 13528-1-AP (TDRKH specific antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HeLa using 13528-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 13528-1-AP (TDRKH specific antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 13528-1-AP

  Immunofluorescent analysis of Hela cells, using TDRKH antibody 13528-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• Featured Product
• KD/KO Validated

TDRKH specific Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 13528-1-AP

Publications(18)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, RIP, ELISA

Synonyms

TDRKH, Tudor domain-containing protein 2, Tudor and KH domain-containing protein, tudor and KH domain containing, TDRD2

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Tested Applications

• Positive WB detected in: NCCIT cell, mouse brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 13 publications below
• IHC: See 3 publications below
• IF: See 13 publications below
• IP: See 5 publications below
• RIP: See 1 publications below

Product Information

13528-1-AP targets TDRKH specific in WB, IHC, IF/ICC, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, bovine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag4422

  Product name: Recombinant human TDRKH protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-374 aa of BC032690

  Sequence: MSTERTSWTSLSTIQKIALGFGIPASATVAYILYRRYRESREERLTFVGEDDIEIEMRVPQEAVKLIIGRQGANIKQLRKQTGARIDVDTEDVGDERVLLISGFPVQVCKAKAAIHQILTENTPVSEQLSVPQRSVGRIIGRGGETIRSICKASGAKITCDKESEGTLLLSRLIKISGTQKEVAAAKHLILEKVSEDEELRKRIAHSAETRVPRKQPISVRREDMTEPGGAGEPALWKNTSSSMEPTAPLVTPPPKAGGDMAVVVSKEGSWEKPSDDSFQKSEAQAIPEMPMFEIPSPDFSFHADEYLEVYVSASEHPNHFWIQIVGSRSLQLDKLVNEMTQHYENSVPEDLTVHVGDIVAAPLPTNGSWYRAR

• Full Name: tudor and KH domain containing
• Calculated Molecular Weight: 606 aa, 67 kDa
• Observed Molecular Weight: 67 kDa
• GenBank Accession Number: BC032690
• Gene Symbol: TDRKH
• Gene ID (NCBI): 11022
• RRID: AB_2303299
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9Y2W6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Tudor and KH domain-containing protein(TDRKH)Tudor domains are protein modules that mediate protein-protein interactions, potentially by binding to methylated ligands. Both KH and tudor domains are involved in binding RNA or single-strand DNA. Tudor also is a germ cell-specific protein with multiple Tudor domains and is involved in germ plasm formation and germ cell specification. TDRKH is one Tudor protein with a single Tudor domain.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Nat Cell Biol; Ribosomes guide pachytene piRNA formation on long intergenic piRNA precursors.; Authors - Yu H Sun
	WB	Sci Adv; Global ubiquitylation analysis of mitochondria in primary neurons identifies endogenous Parkin targets following activation of PINK1.; Authors - Odetta Antico
mouse	IF, WB	Nat Commun; PNLDC1 is essential for piRNA 3' end trimming and transposon silencing during spermatogenesis in mice.; Authors - Deqiang Ding ; KO Validated
mouse	IF	Dev Cell; TDRD1 phase separation drives intermitochondrial cement assembly to promote piRNA biogenesis and fertility; Authors - Jie Gao
mouse	IP	EMBO J; MIWI prevents aneuploidy during meiosis by cleaving excess satellite RNA.; Authors - Chia-Ling Hsieh
mouse	WB,IF	EMBO J; Tdrkh is essential for spermatogenesis and participates in primary piRNA biogenesis in the germline.; Authors - Jonathan P Saxe ; KO Validated