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  • KD/KO Validated

TDRKH specific Polyclonal antibody

TDRKH specific Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat and More (1)





Cat no : 13528-1-AP


TDRD2, TDRKH, TDRKH specific, tudor and KH domain containing

Tested Applications

Positive WB detected inNCCIT cell, mouse brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13528-1-AP targets TDRKH specific in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TDRKH specific fusion protein Ag4422
Full Name tudor and KH domain containing
Calculated Molecular Weight 606 aa, 67 kDa
Observed Molecular Weight 67 kDa
GenBank Accession NumberBC032690
Gene Symbol TDRKH
Gene ID (NCBI) 11022
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Tudor and KH domain-containing protein(TDRKH)Tudor domains are protein modules that mediate protein-protein interactions, potentially by binding to methylated ligands. Both KH and tudor domains are involved in binding RNA or single-strand DNA. Tudor also is a germ cell-specific protein with multiple Tudor domains and is involved in germ plasm formation and germ cell specification. TDRKH is one Tudor protein with a single Tudor domain.


Product Specific Protocols
WB protocol for TDRKH specific antibody 13528-1-APDownload protocol
IHC protocol for TDRKH specific antibody 13528-1-APDownload protocol
IF protocol for TDRKH specific antibody 13528-1-APDownload protocol
IP protocol for TDRKH specific antibody 13528-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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Ribosomes guide pachytene piRNA formation on long intergenic piRNA precursors.

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Tdrkh is essential for spermatogenesis and participates in primary piRNA biogenesis in the germline.

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Proc Natl Acad Sci U S A

Mouse Piwi interactome identifies binding mechanism of Tdrkh Tudor domain to arginine methylated Miwi.

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