TDP-43 Monoclonal antibody

TDP-43 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat, Mouse and More (2)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1G4F5

Cat no : 66734-1-Ig

Synonyms

ALS10, TAR DNA binding protein, TAR DNA binding protein 43, TARDBP, TDP 43, TDP43, tdp-43



Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, Jurkat cells, HSC-T6 cells, C6 cells, NIH/3T3 cells
Positive IHC detected inmouse brain tissue, rat liver tissue, human gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66734-1-Ig targets TDP-43 in WB, IF, IHC, ELISA applications and shows reactivity with Human, Rat, Mouse samples.

Tested Reactivity Human, Rat, Mouse
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen fusion protein
Full Name TAR DNA binding protein
Calculated Molecular Weight 43 kDa
Observed Molecular Weight 43 kDa
GenBank Accession NumberBC001487
Gene Symbol TDP-43
Gene ID (NCBI) 23435
RRIDAB_2882084
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Transactivation response (TAR), DNA-binding protein of 43 kDa (also known as TARDBP or TDP-43), was first isolated as a transcriptional inactivator binding to the TAR DNA element of the HIV-1 virus. Neumann et al. (2006) found that a hyperphosphorylated, ubiquitinated, and cleaved form of TARDBP, known as pathologic TDP-43, is the major component of the tau-negative and ubiquitin-positive inclusions that characterize amyotrophic lateral sclerosis (ALS) and the most common pathological subtype of frontotemporal lobar degeneration (FTLD-U). 66734-1-Ig is a mouse monoclonal antibody recognizing the intact protein of TDP-43.

Protocols

Product Specific Protocols
WB protocol for TDP-43 antibody 66734-1-IgDownload protocol
IHC protocol for TDP-43 antibody 66734-1-IgDownload protocol
FC protocol for TDP-43 antibody 66734-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Acta Neuropathol

Neurons selectively targeted in frontotemporal dementia reveal early stage TDP-43 pathobiology.

Authors - Alissa L Nana
mouseWB, IF

Int J Biol Sci

Spreading of pathological TDP-43 along corticospinal tract axons induces ALS-like phenotypes in Atg5+/- mice.

Authors - Rui Zhang
mouseIHC

Exp Neurol

Muscle-dominant wild-type TDP-43 expression induces myopathological changes featuring tubular aggregates and TDP-43-positive inclusions.

Authors - Nozomu Tawara
mouseIF

Sci Rep

A microfluidic approach to rescue ALS motor neuron degeneration using rapamycin.

Authors - Phaneendra Chennampally
human

Nat Commun

TDP-43 proteinopathy in ALS is triggered by loss of ASRGL1 and associated with HML-2 expression

Authors - Marta Garcia-Montojo