TDP-43 Monoclonal antibody

TDP-43 Monoclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat, Mouse and More (2)

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

4

CloneNo.

1G4F5

Cat no : 66734-1-Ig

Synonyms

ALS10, TAR DNA binding protein, TAR DNA binding protein 43, TARDBP, TDP 43, TDP43, tdp-43

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Validation Data Gallery

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  WB analysis using 66734-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66734-1-Ig (TDP-43 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 66734-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66734-1-Ig (TDP-43 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 66734-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66734-1-Ig (TDP-43 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 66734-1-Ig

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66734-1-Ig (TDP-43 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 66734-1-Ig

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66734-1-Ig (TDP-43 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat liver using 66734-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 66734-1-Ig (TDP-43 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat liver using 66734-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 66734-1-Ig (TDP-43 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of SH-SY5Y using 66734-1-Ig

  1X10^6 SH-SY5Y cells were intracellularly stained with 0.20 ug/test Anti-Human TDP-43 (66734-1-Ig, Clone:1G4F5) (red) or 0.20 ug control antibody (blue) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000. Fixed with90% MeOH.

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, Jurkat cells, HSC-T6 cells, C6 cells, NIH/3T3 cells
• Positive IHC detected in: mouse brain tissue, rat liver tissue, human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC detected in: SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 3 publications below

Product Information

66734-1-Ig targets TDP-43 in WB, IHC, IF, FC, ELISA applications and shows reactivity with Human, Rat, Mouse samples.

• Tested Reactivity: Human, Rat, Mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: fusion protein
• Full Name: TAR DNA binding protein
• Calculated Molecular Weight: 43 kDa
• Observed Molecular Weight: 43 kDa
• GenBank Accession Number: BC001487
• Gene Symbol: TARDBP
• Gene ID (NCBI): 23435
• RRID: AB_2882084
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Transactivation response (TAR), DNA-binding protein of 43 kDa (also known as TARDBP or TDP-43), was first isolated as a transcriptional inactivator binding to the TAR DNA element of the HIV-1 virus. Neumann et al. (2006) found that a hyperphosphorylated, ubiquitinated, and cleaved form of TARDBP, known as pathologic TDP-43, is the major component of the tau-negative and ubiquitin-positive inclusions that characterize amyotrophic lateral sclerosis (ALS) and the most common pathological subtype of frontotemporal lobar degeneration (FTLD-U). 66734-1-Ig is a mouse monoclonal antibody recognizing the intact protein of TDP-43.

Publications

Species	Application	Title
human	IF	Acta Neuropathol; Neurons selectively targeted in frontotemporal dementia reveal early stage TDP-43 pathobiology.; Authors - Alissa L Nana
mouse	WB, IF	Int J Biol Sci; Spreading of pathological TDP-43 along corticospinal tract axons induces ALS-like phenotypes in Atg5+/- mice.; Authors - Rui Zhang
mouse	IHC	Exp Neurol; Muscle-dominant wild-type TDP-43 expression induces myopathological changes featuring tubular aggregates and TDP-43-positive inclusions.; Authors - Nozomu Tawara
mouse	IF	Sci Rep; A microfluidic approach to rescue ALS motor neuron degeneration using rapamycin.; Authors - Phaneendra Chennampally