TDP-43 Monoclonal antibody
TDP-43 Monoclonal Antibody for WB, IHC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, Rat, Mouse and More (2)
Applications
WB, IF, IHC, ELISA
Conjugate
Unconjugated
CloneNo.
1G4F5
Cat no : 66734-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells, HEK-293 cells, Jurkat cells, HSC-T6 cells, C6 cells, NIH/3T3 cells |
Positive IHC detected in | mouse brain tissue, rat liver tissue, human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 1 publications below |
IHC | See 1 publications below |
IF | See 3 publications below |
Product Information
66734-1-Ig targets TDP-43 in WB, IF, IHC, ELISA applications and shows reactivity with Human, Rat, Mouse samples.
Tested Reactivity | Human, Rat, Mouse |
Cited Reactivity | human, mouse |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | fusion protein |
Full Name | TAR DNA binding protein |
Calculated Molecular Weight | 43 kDa |
Observed Molecular Weight | 43 kDa |
GenBank Accession Number | BC001487 |
Gene Symbol | TDP-43 |
Gene ID (NCBI) | 23435 |
RRID | AB_2882084 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Transactivation response (TAR), DNA-binding protein of 43 kDa (also known as TARDBP or TDP-43), was first isolated as a transcriptional inactivator binding to the TAR DNA element of the HIV-1 virus. Neumann et al. (2006) found that a hyperphosphorylated, ubiquitinated, and cleaved form of TARDBP, known as pathologic TDP-43, is the major component of the tau-negative and ubiquitin-positive inclusions that characterize amyotrophic lateral sclerosis (ALS) and the most common pathological subtype of frontotemporal lobar degeneration (FTLD-U). 66734-1-Ig is a mouse monoclonal antibody recognizing the intact protein of TDP-43.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for TDP-43 antibody 66734-1-Ig | Download protocol |
IHC protocol for TDP-43 antibody 66734-1-Ig | Download protocol |
FC protocol for TDP-43 antibody 66734-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Acta Neuropathol Neurons selectively targeted in frontotemporal dementia reveal early stage TDP-43 pathobiology. | ||
Int J Biol Sci Spreading of pathological TDP-43 along corticospinal tract axons induces ALS-like phenotypes in Atg5+/- mice. | ||
Exp Neurol Muscle-dominant wild-type TDP-43 expression induces myopathological changes featuring tubular aggregates and TDP-43-positive inclusions. | ||
Nat Commun TDP-43 proteinopathy in ALS is triggered by loss of ASRGL1 and associated with HML-2 expression |