Tested Applications
| Positive WB detected in | HeLa cells, HEK-293 cells, HepG2 cells, Y79 cells, SH-SY5Y cells, Neuro-2a cells, PC-12 cells |
| Positive IHC detected in | mouse brain tissue, human gliomas tissue, rat brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | rat brain tissue |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:2000-1:20000 |
| Immunofluorescence (IF)-P | IF-P : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67345-1-Ig targets TDP-43 (C-terminal) in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | TAR DNA binding protein |
| Calculated Molecular Weight | 43 kDa |
| Observed Molecular Weight | 43-45 kDa |
| GenBank Accession Number | NM_007375 |
| Gene Symbol | TDP-43 |
| Gene ID (NCBI) | 23435 |
| RRID | AB_2882603 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q13148 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Transactivation response (TAR), DNA-binding protein of 43 kDa (also known as TARDBP or TDP-43), was first isolated as a transcriptional inactivator binding to the TAR DNA element of the HIV-1 virus. Neumann et al. (2006) found that a hyperphosphorylated, ubiquitinated, and cleaved form of TARDBP, known as pathologic TDP-43, is the major component of the tau-negative and ubiquitin-positive inclusions that characterize amyotrophic lateral sclerosis (ALS) and the most common pathological subtype of frontotemporal lobar degeneration (FTLD-U). 67345-1-Ig is a mouse monoclonal antibody raised against the C-terminal region of human TDP-43.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for TDP-43 (C-terminal) antibody 67345-1-Ig | Download protocol |
| IHC protocol for TDP-43 (C-terminal) antibody 67345-1-Ig | Download protocol |
| WB protocol for TDP-43 (C-terminal) antibody 67345-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-TDP-43 antibody (Cat# 67345-1-Ig) has 15 citations across journals including Molecular Neurodegeneration, Acta Neuropathologica, PNAS, Neurobiology of Disease, FASEB Journal, Neuropharmacology, Frontiers in Molecular Neuroscience, ASN Neuro, Biomedicines, Neurochemistry International, Biosensors, Discover Immunology, Neuroscience Bulletin, and bioRxiv. Research fields center on TDP-43 proteinopathies, ALS, Alzheimer's disease, and frontotemporal dementia, with applications in IF, WB, and IHC.
| Species | Application | Title |
|---|---|---|
Mol Neurodegener Extracellular vesicles in TDP-43 proteinopathies: pathogenesis and biomarker potential | ||
Acta Neuropathol TDP-43 dysregulation impairs cholesterol metabolism linked with myelination defects. | ||
Proc Natl Acad Sci U S A Ubiquitin-specific peptidase-19 links TDP-43 aggregation to ER stress. | ||
Biosensors (Basel) Development of a Sensitive and Reliable Meso Scale Discovery-Based Electrochemiluminescence Immunoassay to Quantify TDP-43 in Human Biofluids | ||
Neurobiol Dis Casein kinase 1 inhibitor avoids TDP-43 pathology propagation in a patient-derived cellular model of amyotrophic lateral sclerosis | ||
Neurobiol Dis CHCHD10S59L/+ mouse model: Behavioral and neuropathological features of frontotemporal dementia |
