TDG / Thymine-DNA glycosylase antibody (pAb)
Host / Isotype
Rabbit / IgG
Reactivity
Mouse
Applications
WB
Cat No : 61437,61438 61437
Synonyms
Validation Data Gallery
Product Information
| Tested Applications |
WB
Applications Validated by Active Motif: WB: 1:500 - 1:1,500 dilution |
| Tested Reactivity | Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | This antibody was raised against a full-length recombinant protein corresponding to mouse thymine DNA glycosylase (TDG). |
| Full Name | TDG / Thymine-DNA glycosylase antibody (pAb) |
| Synonyms | tdg / thymine-dna glycosylase, tdg, thimine, dna, G/T Mismatch-Specific Thymine DNA Glycosylaseglycosylase, western blotting, antibody, polyclonal, wb, pAb, sample |
| Molecular weight | 55 kDa |
| GenBank accession number | NP_766140 |
| RRID | AB_2793636 |
| Purification Method | Protein A Chromatography |
| Buffer | Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
| Storage | Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage. |
Background Information
In the DNA of higher eukaryotes, hydrolytic deamination of 5-methylcytosine to thymine leads to the formation of G/T mismatches. In addition, TET proteins oxidize 5- methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC), 5- formylcytosine (5fC), and 5-carboxylcytosine (5caC). TDG / Thymine-DNA glycosylase functions to correct G/T mispairs to G/C pairs and plays a role in active DNA demethylation by recognizing 5fC and 5caC and converting these to cystosine through the base excision repair (BER) pathway.