TCEB2/Elongin-B Polyclonal antibody

TCEB2/Elongin-B Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse





Cat no : 10779-1-AP


ELOB, Elongin 18 kDa subunit, Elongin B, SIII, SIII p18, TCEB2

Tested Applications

Positive WB detected inHL-60 cells, mouse skeletal muscle tissue, HEK-293 cells, HeLa cells, K-562 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 4 publications below

Product Information

10779-1-AP targets TCEB2/Elongin-B in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TCEB2/Elongin-B fusion protein Ag1228
Full Name transcription elongation factor B (SIII), polypeptide 2 (18kDa, elongin B)
Calculated Molecular Weight 13 kDa
Observed Molecular Weight 18 kDa
GenBank Accession NumberBC013306
Gene Symbol TCEB2
Gene ID (NCBI) 6923
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TCEB2 encodes the protein elongin B, which is a subunit of the transcription factor B (SIII) complex and is also reported to function as an adapter protein in the proteasomal degradation of target proteins via different E3 ubiquitin ligase complexes (PMID: 26531153). TCEB2 has 2 isoforms with the molecular mass of 13 and 18 kDa.


Product Specific Protocols
WB protocol for TCEB2/Elongin-B antibody 10779-1-APDownload protocol
IHC protocol for TCEB2/Elongin-B antibody 10779-1-APDownload protocol
IP protocol for TCEB2/Elongin-B antibody 10779-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Cell Death Differ

CRL2-KLHDC3 E3 ubiquitin ligase complex suppresses ferroptosis through promoting p14ARF degradation.

Authors - Pingzhao Zhang

Cell Signal

pVHL interacts with Ceramide kinase like (CERKL) protein and ubiquitinates it for oxygen dependent proteasomal degradation.

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