TBX6 Polyclonal antibody

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  WB analysis of HeLa using 12447-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12447-1-AP (TBX6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 12447-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 12447-1-AP (TBX6 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of A375 using 12447-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 12447-1-AP (TBX6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 12447-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 12447-1-AP (TBX6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 12447-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 12447-1-AP (TBX6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 12447-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 12447-1-AP (TBX6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human lung using 12447-1-AP

  human lung tissue were subjected to SDS PAGE followed by western blot with 12447-1-AP (TBX6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of human placenta using 12447-1-AP

  IP result of anti-TBX6 (IP:12447-1-AP, 4ug; Detection:12447-1-AP 1:500) with human placenta tissue lysate 2800ug.

TBX6 Polyclonal Antibody for WB, IP, ELISA

Cat No. 12447-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, ELISA

Synonyms

DFNB67, T box 6, T box protein 6, TBX6

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Tested Applications

• Positive WB detected in: HeLa cells, A375 cells, A549 cells, human lung tissue, human placenta tissue, mouse lung tissue, Raji cells
• Positive IP detected in: human placenta tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

12447-1-AP targets TBX6 in WB, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag3050

  Product name: Recombinant human TBX6 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 87-436 aa of BC026031

  Sequence: SLPGVSLSLENRELWKEFSSVGTEMIITKAGRRMFPACRVSVTGLDPEARYLFLLDVIPVDGARYRWQGRRWEPSGKAEPRLPDRVYIHPDSPATGAHWMRQPVSFHRVKLTNSTLDPHGHLILHSMHKYQPRIHLVRAAQLCSQHWGGMASFRFPETTFISVTAYQNPQITQLKIAANPFAKGFRENGRNCKRERDARVKRKLRGPEPAATEAYGSGDTPGGPCDSTLGGDIRESDPEQAPAPGEATAAPAPLCGGPSAEAYLLHPAAFHGAPSHLPTRSPSFPEAPDSGRSAPYSAAFLELPHGSGGSGYPAAPPAVPFAPHFLQGGPFPLPYTAPGGYLDVGSKPMY

• Full Name: T-box 6
• Calculated Molecular Weight: 436 aa, 47 kDa
• Observed Molecular Weight: 58 kDa
• GenBank Accession Number: BC026031
• Gene Symbol: TBX6
• Gene ID (NCBI): 6911
• RRID: AB_2200836
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O95947
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TBX6, also named as T-box transcription factor TBX6, is a 436 amino acid protein, which localizes in the nucleus and forms a dimeric complex with DNA. T-box transcription factor that plays an essential role in the determination of the fate of axial stem cells: neural vs mesodermal. The calculated molecular weight of TBX6 is 47 kDa, but the modified TBX6 is about 58 kDa.