TBX21,T-bet Recombinant antibody

TBX21,T-bet Uni-rAb^TM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240849C1

Cat no : 83804-6-RR

Synonyms

TBX21, TBX 21, T-box transcription factor TBX21, T-box protein 21, T bet

---

Validation Data Gallery

• 

  WB analysis of CTLL-2 using 83804-6-RR

  CTLL-2 cells were subjected to SDS PAGE followed by western blot with 83804-6-RR (TBX21,T-bet antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  Affinity and Kinetic Characterization of 83804-6-RR

  Biolayer interferometry (BLl) kinetic assays of 83804-6-RR against Human TBX21,T-bet were performed. The affinity constant is below 1 pM.

---

Tested Applications

• Positive WB detected in: CTLL-2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83804-6-RR targets TBX21,T-bet in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: TBX21,T-bet fusion protein Ag4618
• Full Name: T-box 21
• Calculated Molecular Weight: 535 aa, 58 kDa
• Observed Molecular Weight: 60-65 kDa
• GenBank Accession Number: BC039739
• Gene Symbol: TBX21/T-bet
• Gene ID (NCBI): 30009
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TBX21 is a transcription factor that drives the Th1 immune response primarily through promoting expression of the IFN-γ gene. TBX21 initiates TH1 lineage development from naive TH precursor cells both by activating TH1 genetic programs and by repressing the opposing TH2 programs. It has also been shown to play an influential role in inflammatory processes such as inflammatory bowel disease, autoimmune diseases such as rheumatoid arthritis, and immune-mediated conditions like asthma.