TBP Polyclonal antibody

TBP Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 22246-1-AP

Synonyms

GTF2D, GTF2D1, SCA17, TATA binding factor, TATA box binding protein, TATA box factor, TATA sequence binding protein, TBP, TF2D, TFIID

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Validation Data Gallery

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  WB analysis of Jurkat using 22246-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 22246-1-AP (TBP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 22246-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 22246-1-AP (TBP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SKOV-3 using 22246-1-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 22246-1-AP (TBP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 22246-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 22246-1-AP (TBP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse colon using 22246-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 22246-1-AP (TBP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of MCF-7 using 22246-1-AP

  Immunofluorescent analysis of MCF-7 cells, using TBP antibody 22246-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: Jurkat cells, COLO 320 cells, HeLa cells, mouse colon tissue, SKOV-3 cells
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

The immunogen of 22246-1-AP is TBP Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TBP fusion protein Ag17740
• Full Name: TATA box binding protein
• Calculated Molecular Weight: 338 aa, 38 kDa
• Observed Molecular Weight: mouse/rat 33-36 kDa and human 37-43kDa
• GenBank Accession Number: BC110341
• Gene Symbol: TBP
• Gene ID (NCBI): 6908
• RRID: AB_2879046
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TBP, TATA box-binding protein, is a DNA-binding subunit of TFIID, which is a multisubunit complex essential for the expression of most protein-encoding genes. The well-conserved C-terminal domains of 180 aa is both necessary and sufficient for interaction with DNA and for assembly of the basal transcription apparatus.The N terminus of TBP modulates the DNA-binding activity of the C terminus of the protein. Once binds to the TATA box mediated by TBP, TFIID initiate the transcription step of the pre-initiation complex(PIC), playing a role in the activation of eukaryotic genes transcribed by RNA polymerase II. This is a rabbit polyclonal antibody raised against part of N-terminal chain of human TBP, and specially recognize a ~46 kDa band.

Publications

Species	Application	Title
mouse	WB	Breast Cancer; Isoforms of IDH in breast carcinoma: IDH2 as a potent prognostic factor associated with proliferation in estrogen-receptor positive cases.; Authors - Hiroyuki Minemura
human	WB	Mol Med Rep; Subcellular localization of DJ-1 in human HL-60 leukemia cells in response to diallyl disulfide treatment.; Authors - Qingye Li

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