TBCB Polyclonal antibody

TBCB Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 15782-1-AP

Synonyms

CG22, CKAP1, CKAPI, TBCB, tubulin folding cofactor B, Tubulin specific chaperone B

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Validation Data Gallery

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  WB analysis using 15782-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15782-1-AP (TBCB antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 15782-1-AP

  IP result of anti-TBCB (IP:15782-1-AP, 3ug; Detection:15782-1-AP 1:500) with HeLa cells lysate 1200ug.

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  IF Staining of HeLa using 15782-1-AP

  Immunofluorescent analysis of Hela cells, using TBCB antibody 15782-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  IF Staining of HeLa using 15782-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using TBCB antibody (15782-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: A431 cells, HeLa cells, NCI-H1299 cells, Neuro-2a cells, mouse brain tissue, rat brain tissue
• Positive IP detected in: HeLa cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

15782-1-AP targets TBCB in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TBCB fusion protein Ag8473
• Full Name: tubulin folding cofactor B
• Calculated Molecular Weight: 244 aa, 27 kDa
• Observed Molecular Weight: 32 kDa
• GenBank Accession Number: BC005969
• Gene Symbol: TBCB
• Gene ID (NCBI): 1155
• RRID: AB_2302986
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	IF	Histochem Cell Biol; Spatio-temporal distribution of tubulin-binding cofactors and posttranslational modifications of tubulin in the cochlea of mice.; Authors - Lukas Juergens