TBC1D20 Polyclonal antibody

TBC1D20 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 16464-1-AP

Synonyms

C20orf140, TBC1 domain family member 20, TBC1 domain family, member 20, TBC1D20

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Validation Data Gallery

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  WB analysis using 16464-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16464-1-AP (TBC1D20 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 16464-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16464-1-AP (TBC1D20 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 16464-1-AP

  WB result of TBC1D20 antibody (16464-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TBC1D20 transfected A549 cells.

• 

  WB analysis of A549 using 16464-1-AP

  WB result of TBC1D20 antibody (16464-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TBC1D20 transfected A549 cells.

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Tested Applications

• Positive WB detected in: HepG2 cells, A549 cells, LNCaP cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16464-1-AP targets TBC1D20 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TBC1D20 fusion protein Ag9539
• Full Name: TBC1 domain family, member 20
• Calculated Molecular Weight: 403 aa, 46 kDa
• Observed Molecular Weight: 38-40 kDa
• GenBank Accession Number: BC014983
• Gene Symbol: TBC1D20
• Gene ID (NCBI): 128637
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TBC1D20 belongs to a family of GTPase activator proteins of Rab-like small GTPases.TBC1D20 is an endoplasmic reticulum (ER) type II membrane protein with the TBC domain positioned in the cytosol. Depletion of this protein inhibits replication of the virus and HCV infection. Mutations in this gene are associated with Warburg micro syndrome 4.