TAF1C Polyclonal antibody

TAF1C Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13341-1-AP

Synonyms

SL1, TAF1C, TAFI110, TAFI95, TBP associated factor 1C

---

Validation Data Gallery

• 

  WB analysis of HT-1080 using 13341-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 13341-1-AP (TAF1C antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 13341-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13341-1-AP (TAF1C antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 13341-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13341-1-AP (TAF1C antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive WB detected in: HT-1080 cells
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13341-1-AP targets TAF1C in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TAF1C fusion protein Ag4164
• Full Name: TATA box binding protein (TBP)-associated factor, RNA polymerase I, C, 110kDa
• Calculated Molecular Weight: 869 aa, 95 kDa
• Observed Molecular Weight: 95 kDa
• GenBank Accession Number: BC028131
• Gene Symbol: TAF1C
• Gene ID (NCBI): 9013
• RRID: AB_10646433
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.