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SignalBright Pro Chemiluminescent Substrate

Sensitivity:

Mid to high femtogram

Signal duration:

More than 12 hours

Compatibility:

suitable for PVDF and nitrocellulose membranes

Cat no : PK10011

Validation Data Gallery

  • Serial dilutions of RUVBL primary antibody (10210-2-AP) <br>Primary: Proteintech RUVBL1 (10210-2-AP) at various dilutions (see image) <br>Secondary: Proteintech HRP-conjugated Affinipure Goat Anti-Rabbit IgG (SA00001-2); 1:6,000 <br>Exposure time: 1 min <br>Chemiluminescent substrates from leading competitor, SignalBright Pro (PK10011), SignalBright Max (PK10013)

    Serial dilutions of RUVBL primary antibody (10210-2-AP)
    Primary: Proteintech RUVBL1 (10210-2-AP) at various dilutions (see image)
    Secondary: Proteintech HRP-conjugated Affinipure Goat Anti-Rabbit IgG (SA00001-2); 1:6,000
    Exposure time: 1 min
    Chemiluminescent substrates from leading competitor, SignalBright Pro (PK10011), SignalBright Max (PK10013)

  • Serial dilutions of HEK-293 cell lysates <br>Primary: Proteintech RUVBL1 (10210-2-AP); 1:8,000 <br>Secondary: Proteintech HRP-conjugated Affinipure Goat Anti-Rabbit IgG (SA00001-2); 1:6,000 <br>Exposure time: 1 min <br>Chemiluminescent substrates from leading competitor, SignalBright Pro (PK10011), SignalBright Max (PK10013)

    Serial dilutions of HEK-293 cell lysates
    Primary: Proteintech RUVBL1 (10210-2-AP); 1:8,000
    Secondary: Proteintech HRP-conjugated Affinipure Goat Anti-Rabbit IgG (SA00001-2); 1:6,000
    Exposure time: 1 min
    Chemiluminescent substrates from leading competitor, SignalBright Pro (PK10011), SignalBright Max (PK10013)

Product Information

SignalBright Pro is a luminol-based, chemiluminescent substrate for HRP (horseradish peroxidase) which provides a much higher sensitivity over traditional ECL reagents. SignalBright Pro provides mid to high- femtogram detection levels in Western Blot and ELISA analysis, with long lasting signal up to 24 hours.

Key Characteristics:

· Sensitivity: Mid to high femtogram

· Signal duration: >12 hours

· Compatibility: suitable for PVDF and nitrocellulose membranes

· Comparable sensitivity to SuperSignal™ West Dura.

Instructions for Use

SignalBright Pro Chemiluminescent Detection is prepared by combining a 1:1 ratio of the Chemiluminescent Substrate Solution  and   Stable Peroxide Solution  to create a working solution. Allow the working solution to come to room temperature before applying to the membrane, make sure this is done in darkness. Apply a minimum of 0.1ml of the working solution per membrane cm2. Leave 5 minutes for signal to develop prior to imaging.

· Recommended primary antibody concentration: 0.02-1 ug/ml

· Recommended HRP-conjugated secondary antibody concentration: 4-20 ng/ml

Product Information

Cat.SizeContent
PK1001120mL10mL Chemiluminescent Substrate Solution
10mL Stable Peroxide Solution
100mL50mL Chemiluminescent Substrate Solution
50mL Stable Peroxide Solution

Storage

Store at 4ºC. Expires 1 year after purchase.

Cited in Article as

PK10011, SignalBright Pro Chemiluminescent Substrate, Proteintech, IL, USA