CoraLite® Plus 750-conjugated Shh Recombinant antibody

Shh Recombinant Antibody for WB

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB

Conjugate

CoraLite® Plus 750 Fluorescent Dye

CloneNo.

240057E7

Cat no : CL750-83280

Synonyms

Hxl3, Hx, Hhg1, Dsh, 9530036O11Rik

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Validation Data Gallery

• 

  WB analysis using CL750-83280

  Various lysates were subjected to SDS PAGE followed by western blot with CL750-83280 (Shh antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse liver tissue, rat liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of CL750-83280 is Shh Fusion Protein expressed in E. coli.

• Tested Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: sonic hedgehog
• Calculated Molecular Weight: 48KD
• Observed Molecular Weight: 19-50 kDa
• GenBank Accession Number: NM_009170.3
• Gene Symbol: Shh
• Gene ID (NCBI): 20423
• Conjugate: CoraLite® Plus 750 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 755 nm / 780 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.