Sec8 Polyclonal antibody, PBS Only

Sec8 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 11913-1-PBS

Synonyms

SEC8L1, REC8, KIAA1699, Exocyst complex component Sec8, Exocyst complex component 4

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Validation Data Gallery

• 

  WB analysis of Jurkat using 11913-1-AP (same clone as 11913-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11913-1-AP (Sec8 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11913-1-PBS in a different storage buffer formulation.

• 

  WB analysis of U-251 using 11913-1-AP (same clone as 11913-1-PBS)

  U-251 cells were subjected to SDS PAGE followed by western blot with 11913-1-AP (Sec8 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11913-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse testis using 11913-1-AP (same clone as 11913-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 11913-1-AP (Sec8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11913-1-PBS in a different storage buffer formulation.

• 

  IP experiment of Jurkat using 11913-1-AP (same clone as 11913-1-PBS)

  IP result of anti-Sec8 (IP:11913-1-AP, 4ug; Detection:11913-1-AP 1:300) with Jurkat cells lysate 2800ug. This data was developed using the same antibody clone with 11913-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 11913-1-AP (same clone as 11913-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11913-1-AP (Sec8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11913-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 11913-1-AP (same clone as 11913-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11913-1-AP (Sec8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11913-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 11913-1-AP (same clone as 11913-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11913-1-AP (Sec8 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11913-1-PBS in a different storage buffer formulation.

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Product Information

11913-1-PBS targets Sec8 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Sec8 fusion protein Ag2514
• Full Name: exocyst complex component 4
• Calculated Molecular Weight: 110 kDa
• Observed Molecular Weight: 110-120 kDa
• GenBank Accession Number: BC026174
• Gene Symbol: Sec8
• Gene ID (NCBI): 60412
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.