SYNJ2BP Polyclonal antibody

• Featured Product
• KD/KO Validated

SYNJ2BP Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

11

Cat no : 15666-1-AP

Synonyms

Synaptojanin-2-binding protein, OMP25, ARIP2

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Validation Data Gallery

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  WB analysis using 15666-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15666-1-AP (SYNJ2BP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 15666-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 15666-1-AP (SYNJ2BP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse lung using 15666-1-AP

  IP result of anti-SYNJ2BP (IP:15666-1-AP, 3ug; Detection:15666-1-AP 1:500) with mouse lung tissue lysate 4000ug.

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  IHC staining of human stomach cancer using 15666-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 15666-1-AP (SYNJ2BP antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human stomach cancer using 15666-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 15666-1-AP (SYNJ2BP antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of MCF-7 using 15666-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using SYNJ2BP antibody (15666-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HepG2 using 15666-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human SYNJ2BP (15666-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: mouse kidney tissue, A549 cells, rat kidney tissue, mouse lung tissue
• Positive IP detected in: mouse lung tissue
• Positive IHC detected in: human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 10 publications below
• IHC: See 3 publications below
• IF: See 2 publications below
• IP: See 1 publications below
• CoIP: See 2 publications below

Product Information

15666-1-AP targets SYNJ2BP in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SYNJ2BP fusion protein Ag8130
• Full Name: synaptojanin 2 binding protein
• Calculated Molecular Weight: 145 aa, 16 kDa
• Observed Molecular Weight: 16 kDa
• GenBank Accession Number: BC007704
• Gene Symbol: SYNJ2BP
• Gene ID (NCBI): 55333
• RRID: AB_2201149
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Activin, a member of the TGF-beta superfamily, plays important roles in reproductive tissues as a stimulator of follicle-stimulating hormone (FSH) secretion and inhibits the proliferation of breast cancer cells. Activin receptor-interacting protein 2 (ARIP2), also known as synaptojanin-2-binding protein (SYNJ2BP), is located in the outer membrane of mitochondria. ARIP2 is widely distributed in various human tissues, and has been recently identified in mouse tissues as a regulatory protein of activin signal transduction by interacting with activin type II receptors. ARIP2 may be a putative growth-promoting factor involved in breast tumorigenesis and tumor development. A double band of size 15.9 kDa can be detected in Western blot (PMID: 23284606).

Publications

Species	Application	Title
human	WB	Science; The endoplasmic reticulum P5A-ATPase is a transmembrane helix dislocase.; Authors - Michael J McKenna
	IF	Neuron; Neuronal mitochondria transport Pink1 mRNA via synaptojanin 2 to support local mitophagy.; Authors - Angelika B Harbauer
human	WB	Cell Rep; Sam50 Regulates PINK1-Parkin-Mediated Mitophagy by Controlling PINK1 Stability and Mitochondrial Morphology.; Authors - Fenglei Jian
human,mouse	WB,IHC,IP	Oncotarget; SYNJ2BP promotes the degradation of PTEN through the lysosome-pathway and enhances breast tumor metastasis via PI3K/AKT/SNAI1 signaling.; Authors - Miao Wang ; KD Validated
human	WB	J Cell Sci; Mitochondrial antiviral-signalling protein is a client of the BAG6 protein quality control complex.; Authors - Peristera Roboti
human	WB,IHC	J Exp Clin Cancer Res; SYNJ2BP inhibits tumor growth and metastasis by activating DLL4 pathway in hepatocellular carcinoma.; Authors - Xiao Liu