STT3A Polyclonal antibody

• Featured Product
• KD/KO Validated

STT3A Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

21

Reviews (2)

Cat no : 12034-1-AP

Synonyms

STT3-A, ITM1, Integral membrane protein 1, EC:2.4.99.18, Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3A

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Validation Data Gallery

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  WB analysis of HepG2 using 12034-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12034-1-AP (STT3A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 12034-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 12034-1-AP (STT3A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human stomach using 12034-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 12034-1-AP (STT3A antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach using 12034-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 12034-1-AP (STT3A antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 12034-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 12034-1-AP (STT3A antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HepG2 cells, K-562 cells
• Positive IHC detected in: human stomach tissue, human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 6 publications below
• WB: See 19 publications below
• IHC: See 3 publications below
• IF: See 1 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Product Information

12034-1-AP targets STT3A in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: STT3A fusion protein Ag2698
• Full Name: STT3, subunit of the oligosaccharyltransferase complex, homolog A (S. cerevisiae)
• Calculated Molecular Weight: 705 aa, 81 kDa
• Observed Molecular Weight: 65-100 kDa
• GenBank Accession Number: BC020965
• Gene Symbol: STT3A
• Gene ID (NCBI): 3703
• RRID: AB_2877818
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

STT3A, also named as Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3A, is a 705 amino acid prtein, which belongs to the STT3 family. STT3A is expressed at high levels in placenta, liver, muscle and pancreas, and at very low levels in brain, lung and kidney. STT3A is a catalytic subunit of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). SST3A seems to be involved in complex substrate specificity. STT3A is present in the majority of OST complexes and mediates cotranslational N-glycosylation of most sites on target proteins, while STT3B-containing complexes are required for efficient post-translational glycosylation and mediate glycosylation of sites that have been skipped by STT3A.

Publications

Species	Application	Title
human	WB,IF,IP	Cancer Discov; Pharmacologic Suppression of B7-H4 Glycosylation Restores Antitumor Immunity in Immune-Cold Breast Cancers.; Authors - Xinxin Song ; KD Validated
human	WB	Nat Chem Biol; Oligosaccharyltransferase inhibition induces senescence in RTK-driven tumor cells.; Authors - Cecilia Lopez-Sambrooks
human	WB,CoIP	Mol Cell Proteomics; Thyroglobulin Interactome Profiling Defines Altered Proteostasis Topology Associated With Thyroid Dyshormonogenesis; Authors - Madison T. Wright
mouse	WB	Cell Mol Gastroenterol Hepatol; Mettl14-Mediated m6A Modification Facilitates Liver Regeneration by Maintaining Endoplasmic Reticulum Homeostasis.; Authors - Xiaoyue Cao
human	WB	MBio; Dengue Virus Hijacks a Noncanonical Oxidoreductase Function of a Cellular Oligosaccharyltransferase Complex.; Authors - David L Lin
human	WB	EBioMedicine; Targeting conserved N-glycosylation blocks SARS-CoV-2 variant infection in vitro.; Authors - Hsiang-Chi Huang ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Parijat (Verified Customer) (09-20-2023)

Good antibody for Western.

• Applications: Western Blot

FH

Sarah-Eve (Verified Customer) (06-02-2023)

Works well

• Applications: Western Blot