STK38 Polyclonal antibody

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• KD/KO Validated

STK38 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 11105-1-AP

Synonyms

Nuclear Dbf2-related kinase 1, Nuclear Dbf2 related kinase 1, NDR1 protein kinase, NDR1, NDR

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Validation Data Gallery

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  WB analysis of HeLa using 11105-1-AP

  WB result of STK38 antibody (11105-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-STK38 transfected HeLa cells.

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  WB analysis using 11105-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11105-1-AP (STK38 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of MCF-7 using 11105-1-AP

  IP result of anti-STK38 (IP:11105-1-AP, 4ug; Detection:11105-1-AP 1:300) with MCF-7 cells lysate 800ug.

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  IHC staining of mouse cerebellum using 11105-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 11105-1-AP (STK38 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 11105-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 11105-1-AP (STK38 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, HepG2 cells, Jurkat cells, MCF-7 cells
• Positive IP detected in: MCF-7 cells
• Positive IHC detected in: mouse cerebellum tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IP: See 1 publications below

Product Information

11105-1-AP targets STK38 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: STK38 fusion protein Ag1559
• Full Name: serine/threonine kinase 38
• Calculated Molecular Weight: 54 kDa
• Observed Molecular Weight: 50-55 kDa
• GenBank Accession Number: BC012085
• Gene Symbol: STK38
• Gene ID (NCBI): 11329
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Serine/threonine-protein kinase 38 belongs to AGC Ser/Thr protein kinase family.STK38 implicates in cell proliferation and/or tumor progression, and might play a role in the HIV-1 life cycle.Researches showed that STK38 with STK38L, were proapoptotic kinases and key members of the RASSF1/STK4 signaling cascade and its kinase activity increased in early mitotic phase and was dependent on FRY and STK3.This antibody recognizes the C-terminal of STK38.

Publications

Species	Application	Title
human	WB,IP	Nat Commun; USP9X regulates centrosome duplication and promotes breast carcinogenesis.; Authors - Xin Li