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STK38 Polyclonal antibody

STK38 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 11105-1-AP

Publications (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, ELISA

Synonyms

Nuclear Dbf2-related kinase 1, Nuclear Dbf2 related kinase 1, NDR1 protein kinase, NDR1, NDR

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

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Filter: AllWBIPIHC

WB analysis of HeLa using 11105-1-AP
WB result of STK38 antibody (11105-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-STK38 transfected HeLa cells.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis using 11105-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11105-1-AP (STK38 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IP experiment of MCF-7 using 11105-1-AP
IP result of anti-STK38 (IP:11105-1-AP, 4ug; Detection:11105-1-AP 1:300) with MCF-7 cells lysate 800ug.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse cerebellum using 11105-1-AP
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 11105-1-AP (STK38 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse cerebellum using 11105-1-AP
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 11105-1-AP (STK38 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information
Publications (1)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	HEK-293 cells, HeLa cells, HepG2 cells, Jurkat cells, MCF-7 cells
Positive IP detected in	MCF-7 cells
Positive IHC detected in	mouse cerebellum tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:3000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11105-1-AP targets STK38 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag1559 Product name: Recombinant human STK38 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 116-465 aa of BC012085 Sequence: AMKILRKADMLEKEQVGHIRAERDILVEADSLWVVKMFYSFQDKLNLYLIMEFLPGGDMMTLLMKKDTLTEEETQFYIAETVLAIDSIHQLGFIHRDIKPDNLLLDSKGHVKLSDFGLCTGLKKAHRTEFYRNLNHSLPSDFTFQNMNSKRKAETWKRNRRQLAFSTVGTPDYIAPEVFMQTGYNKLCDWWSLGVIMYEMLIGYPPFCSETPQETYKKVMNWKETLTFPPEVPISEKAKDLILRFCCEWEHRIGAPGVEEIKSNSFFEGVDWEHIRERPAAISIEIKSIDDTSNFDEFPESDILKPTVATSNHPETDYKNKDWVFINYTYKRFEGLTARGAIPSYMKAAK Predict reactive species
Full Name	serine/threonine kinase 38
Calculated Molecular Weight	54 kDa
Observed Molecular Weight	50-55 kDa
GenBank Accession Number	BC012085
Gene Symbol	STK38
Gene ID (NCBI)	11329
RRID	AB_2196792
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q15208
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Serine/threonine-protein kinase 38 belongs to AGC Ser/Thr protein kinase family.STK38 implicates in cell proliferation and/or tumor progression, and might play a role in the HIV-1 life cycle.Researches showed that STK38 with STK38L, were proapoptotic kinases and key members of the RASSF1/STK4 signaling cascade and its kinase activity increased in early mitotic phase and was dependent on FRY and STK3.This antibody recognizes the C-terminal of STK38.

Protocols

Product Specific Protocols
IHC protocol for STK38 antibody 11105-1-AP	Download protocol
IP protocol for STK38 antibody 11105-1-AP	Download protocol
WB protocol for STK38 antibody 11105-1-AP	Download protocol

Standard Protocols
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Publications

What published studies show

The STK38 Polyclonal antibody (Cat# 11105-1-AP) has 1 citation. It was used in a study published in Nature Communications (2017) investigating how USP9X regulates centrosome duplication and promotes breast carcinogenesis. The antibody was applied in Western blot and immunoprecipitation experiments on human samples, placing its cited use within the fields of cancer biology and cell cycle regulation.