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STK38 Polyclonal antibody

STK38 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Cat no : 11105-1-AP

Synonyms

Nuclear Dbf2-related kinase 1, Nuclear Dbf2 related kinase 1, NDR1 protein kinase, NDR1, NDR



Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells, Jurkat cells, MCF-7 cells
Positive IP detected inMCF-7 cells
Positive IHC detected inmouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11105-1-AP targets STK38 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen STK38 fusion protein Ag1559
Full Name serine/threonine kinase 38
Calculated Molecular Weight 55 kDa
Observed Molecular Weight 50-55 kDa
GenBank Accession NumberBC012085
Gene Symbol STK38
Gene ID (NCBI) 11329
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Serine/threonine-protein kinase 38 belongs to AGC Ser/Thr protein kinase family.STK38 implicates in cell proliferation and/or tumor progression, and might play a role in the HIV-1 life cycle.Researches showed that STK38 with STK38L, were proapoptotic kinases and key members of the RASSF1/STK4 signaling cascade and its kinase activity increased in early mitotic phase and was dependent on FRY and STK3.This antibody recognizes the C-terminal of STK38.

Protocols

Product Specific Protocols
WB protocol for STK38 antibody 11105-1-APDownload protocol
IHC protocol for STK38 antibody 11105-1-APDownload protocol
IP protocol for STK38 antibody 11105-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IP

Nat Commun

USP9X regulates centrosome duplication and promotes breast carcinogenesis.

Authors - Xin Li