STK36 Polyclonal antibody, PBS Only

STK36 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 12559-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of mouse testis using 12559-1-AP (same clone as 12559-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12559-1-AP (STK36 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12559-1-PBS in a different storage buffer formulation.

• 

  IP experiment of mouse testis using 12559-1-AP (same clone as 12559-1-PBS)

  IP result of anti-STK36 (IP:12559-1-AP, 4ug; Detection:12559-1-AP 1:500) with mouse testis tissue lysate 4000ug. This data was developed using the same antibody clone with 12559-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 12559-1-AP (same clone as 12559-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12559-1-AP (STK36 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12559-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 12559-1-AP (same clone as 12559-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12559-1-AP (STK36 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12559-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse stomach using 12559-1-AP (same clone as 12559-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 12559-1-AP (STK36 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12559-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat stomach using 12559-1-AP (same clone as 12559-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 12559-1-AP (STK36 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12559-1-PBS in a different storage buffer formulation.

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Product Information

12559-1-PBS targets STK36 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: STK36 fusion protein Ag3279
• Full Name: serine/threonine kinase 36, fused homolog (Drosophila)
• Calculated Molecular Weight: 1315 aa, 144 kDa
• Observed Molecular Weight: 144 kDa
• GenBank Accession Number: BC026158
• Gene Symbol: STK36
• Gene ID (NCBI): 27148
• RRID: AB_2196785
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

STK36(Serine/threonine-protein kinase 36) is also named as KIAA1278 and belongs to the Ser/Thr protein kinase family. The Serine/threonine protein kinase plays an important role in the sonic hedgehog (Shh) pathway by regulating the activity of GLI transcription factors and controls the activity of the transcriptional regulators GLI1, GLI2 and GLI3 by opposing the effect of SUFU and promoting their nuclear localization. It has 2 isoforms produced by alternative splicing.