STAT6 Polyclonal antibody

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STAT6 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 51073-1-AP Copied!

Publications (55)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ChIP, RIP, ELISA

Synonyms

IL-4 Stat, Signal transducer and activator of transcription 6, STAT 6, STAT6B, STAT6C

• 

  WB analysis of NIH/3T3 using 51073-1-AP

  WB result of STAT6 antibody (51073-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-STAT6 transfected NIH/3T3 cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 51073-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 51073-1-AP (STAT6 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 51073-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 51073-1-AP (STAT6 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human colon cancer using 51073-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 51073-1-AP (STAT6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse lung using 51073-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 51073-1-AP (STAT6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse lung using 51073-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 51073-1-AP (STAT6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human lung using 51073-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 51073-1-AP (STAT6 antibody) at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human lung using 51073-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 51073-1-AP (STAT6 antibody) at dilution of 1:50 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse lung using 51073-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 51073-1-AP (STAT6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse lung using 51073-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 51073-1-AP (STAT6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HeLa cells, NIH/3T3 cells, THP-1 cells, Jurkat cells, PC-12 cells
• Positive IHC detected in: mouse lung tissue, human colon cancer tissue, human lung tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

51073-1-AP targets STAT6 in WB, IHC, IF, ChIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: signal transducer and activator of transcription 6, interleukin-4 induced
• Calculated Molecular Weight: 94 kDa
• Observed Molecular Weight: 100-110 kDa
• GenBank Accession Number: BC075852
• Gene Symbol: STAT6
• Gene ID (NCBI): 6778
• RRID: AB_2197244
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P42226
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

STAT6, also named as Signal transducer and activator of transcription 6, is a 847 amino acid protein, which belongs to the transcription factor STAT family. STAT6 forms a homodimer or a heterodimer with a related family member. STAT6 translocates into the nucleus in response to phosphorylation. STAT6 is Involved in IL4/interleukin-4- and IL3/interleukin-3-mediated signaling. The antibody could identify about 100 kDa band, similar to the reported in the literature(PMID: 10961983).

Publications

What published studies show

Anti-STAT6 (catalog #51073-1-AP) has 55 total citations. Journals include Nature Communications, PNAS, Cancer Research, Cell Reports, Molecular Therapy, Redox Biology, Journal of Ethnopharmacology, Frontiers in Immunology, International Immunopharmacology, and others. Associated research fields span macrophage M1/M2 polarization, oncology, immunology and inflammation, neurology, allergy and asthma, fibrosis, cardiology, infectious disease, and ferroptosis.

Automatically generated from the 55 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
	WB	Cancer Res; Tumor-derived CCL16 Normalizes Tumor Vasculature through Macrophage ICAM-1 Receptor and Enhances Immunotherapy Efficacy in Hepatocellular Carcinoma; Authors - Kunling Chen
		Bone Res; Nuclear FGF2 orchestrates phase separation-mediated rDNA chromatin architecture to control BMSCs cell fate; Authors - Hengguo Zhang
	IHC	Nat Commun; Molecular landscape, subtypes, and therapeutic vulnerabilities of central nervous system solitary fibrous tumors; Authors - Chenhui Zhao
human	WB	Nat Commun; Proteome-wide ligandability maps of drugs with diverse cysteine-reactive chemotypes; Authors - Caiping Tian
mouse	WB	Redox Biol; USP10 protects against pressure overload-induced mitochondrial morphofunctional defects and pathological cardiac hypertrophy through stabilizing cytoplasmic Mfn2; Authors - Runjing Li
human	WB	Cell Death Dis; Ciclopirox drives growth arrest and autophagic cell death through STAT3 in gastric cancer cells; Authors - Lingyan Chen