STAT2 Monoclonal antibody

STAT2 Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat and More (2)

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

4

CloneNo.

1G12C4

Cat no : 66485-1-Ig

Synonyms

ISGF 3, P113, STAT113, STAT2

---

Validation Data Gallery

• 

  WB analysis using 66485-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66485-1-Ig (STAT2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 66485-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66485-1-Ig (STAT2 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of Ramos using 66485-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed Ramos cells using STAT2 antibody (66485-1-Ig, Clone: 1G12C4 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of Ramos using 66485-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed Ramos cells using STAT2 antibody (66485-1-Ig, Clone: 1G12C4 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

---

Tested Applications

• Positive WB detected in: HeLa cells, A431 cells, A549 cells, PC-3 cells, COLO 320 cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: Ramos cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

66485-1-Ig targets STAT2 in WB, IP, IF, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Cited Reactivity: human, mouse, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: STAT2 fusion protein Ag10168
• Full Name: signal transducer and activator of transcription 2, 113kDa
• Calculated Molecular Weight: 851 aa, 98 kDa
• Observed Molecular Weight: 113 kDa
• GenBank Accession Number: BC051284
• Gene Symbol: STAT2
• Gene ID (NCBI): 6773
• RRID: AB_2881851
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

STAT2, also named as p113, belongs to the transcription factor STAT family. It is a signal transducer and activator of transcription that mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize and associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of IF stimulated genes, which drive the cell in an antiviral state. It also interacts with CRSP2, CRSP6, Simian virus 5 protein V, rabies virus phosphoprotein, IFNAR1 and IFNAR2. Its interaction with dengue virus NS5 inhibits the phosphorylation of STAT2, and, when all viral proteins are present (polyprotein), STAT2 is targeted for degradation. The calculated molecular weight of STAT2 is 98 kDa, but phosphorylated STAT2 is about 100-113 kDa.

Publications

Species	Application	Title
human	WB,IF	Int Immunopharmacol; Silencing SIRT1 promotes the anti-HBV action of IFN-α by regulating Pol expression and activating the JAK-STAT signaling pathway; Authors - Qinyan Tang
mouse	WB	Exp Eye Res; Integrated analysis of murine cornea identifies JAK/STAT signaling pathway upregulated specifically in female Vitamin A Deficient mice; Authors - Hanrui Yu
pig	WB,IP	PLoS Pathog; Free ISG15 inhibits Pseudorabies virus infection by positively regulating type I IFN signaling; Authors - Huimin Liu
mouse	WB	J Nanobiotechnology; Apoptotic tumor cell-derived microparticles loading Napabucasin inhibit CSCs and synergistic immune therapy; Authors - Boping Jing