STAMBP Polyclonal antibody

• Featured Product
• KD/KO Validated

STAMBP Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 11346-1-AP

Synonyms

AMSH, STAM binding protein, STAMBP

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Validation Data Gallery

• 

  WB analysis of mouse brain using 11346-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11346-1-AP (STAMBP antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 11346-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 11346-1-AP (STAMBP antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human prostate cancer using 11346-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11346-1-AP (STAMBP antibody) at dilution of 1:100 (under 10x lens).

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Tested Applications

• Positive WB detected in: mouse brain tissue, human placenta tissue
• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below

Product Information

11346-1-AP targets STAMBP in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: STAMBP fusion protein Ag1895
• Full Name: STAM binding protein
• Calculated Molecular Weight: 48 kDa
• Observed Molecular Weight: 48 kDa
• GenBank Accession Number: BC007682
• Gene Symbol: STAMBP
• Gene ID (NCBI): 10617
• RRID: AB_2197509
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Nature; A phosphoinositide signalling pathway mediates rapid lysosomal repair; Authors - Jay Xiaojun Tan
human	WB	J Biol Chem; Endosomal deubiquitinating enzymes control ubiquitination and down-regulation of protease-activated receptor 2.; Authors - Hasdemir Burcu B
human	WB	Mol Biol Cell; Arrestin-2 interacts with the endosomal sorting complex required for transport machinery to modulate endosomal sorting of CXCR4.; Authors - Malik Rohit R ; KD Validated