ST6GALNAC6 Monoclonal antibody, PBS Only

ST6GALNAC6 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

1A7B7

Cat no : 66209-1-PBS

Synonyms

hST6GalNAc VI, RP11 203J24.3, Sialyltransferase 7F, SIAT7 F, SIAT7F, ST6GalNAc VI, ST6GALNAC6, ST6GALNACVI

---

Validation Data Gallery

• 

  WB analysis of COLO 320 using 66209-1-Ig (same clone as 66209-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 66209-1-Ig (ST6GALNAC6 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66209-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse colon using 66209-1-Ig (same clone as 66209-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 66209-1-Ig (ST6GALNAC6 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66209-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse colon using 66209-1-Ig (same clone as 66209-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 66209-1-Ig (ST6GALNAC6 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66209-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skin using 66209-1-Ig (same clone as 66209-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 66209-1-Ig (ST6GALNAC6 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66209-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skin using 66209-1-Ig (same clone as 66209-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 66209-1-Ig (ST6GALNAC6 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66209-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human colon cancer using 66209-1-Ig (same clone as 66209-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using ST6GALNAC6 antibody (66209-1-Ig, Clone: 1A7B7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66209-1-PBS in a different storage buffer formulation.

---

Product Information

66209-1-PBS targets ST6GALNAC6 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: ST6GALNAC6 fusion protein Ag8358
• Full Name: ST6 (alpha-N-acetyl-neuraminyl-2,3-beta-galactosyl-1,3)-N-acetylgalactosaminide alpha-2,6-sialyltransferase 6
• Calculated Molecular Weight: 333aa,38 kDa; 374aa,41 kDa
• Observed Molecular Weight: 38-42 kDa
• GenBank Accession Number: BC007802
• Gene Symbol: ST6GALNAC6
• Gene ID (NCBI): 30815
• RRID: AB_2881600
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -80°C.

Background Information

ST6GALNAC6 is a sialyltransferase which is responsible for the synthesis of all a-series gangliosides. It has a broad expression pattern. ST6GALNAC6 was reported to be a key enzyme in the synthesis of disialyl monosialyl Lewis (Lea), a representative tumor-associated antigen in cancers of the pancreas and colon.