SSPN Polyclonal antibody

SSPN Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 26685-1-AP

Synonyms

DAGA5, Kirsten ras associated protein, KRAG, nanospan, NSPN, Sarcospan, SPN1, SPN2, SSPN

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Validation Data Gallery

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  WB analysis of mouse liver using 26685-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 26685-1-AP (SSPN Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 26685-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 26685-1-AP (SSPN Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse liver tissue, rat liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 26685-1-AP is SSPN Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SSPN fusion protein Ag24511
• Full Name: sarcospan (Kras oncogene-associated gene)
• Calculated Molecular Weight: 243 aa, 27 kDa
• Observed Molecular Weight: 15-20 kDa, 27 kDa
• GenBank Accession Number: BC062299
• Gene Symbol: SSPN
• Gene ID (NCBI): 8082
• RRID: AB_2880602
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.