KRAP/SSFA2 Polyclonal antibody

• Featured Product
• KD/KO Validated

KRAP/SSFA2 Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF-P, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 14157-1-AP

Synonyms

SSFA2, ITPRID2, CS-1, CS1, CS 1

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Validation Data Gallery

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  WB analysis using 14157-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14157-1-AP (KRAP/SSFA2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis using 14157-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14157-1-AP (KRAP/SSFA2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 14157-1-AP

  IP result of anti-KRAP/SSFA2 (IP:14157-1-AP, 4ug; Detection:14157-1-AP 1:500) with mouse testis tissue lysate 4000ug.

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  IHC staining of human stomach using 14157-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 14157-1-AP (KRAP/SSFA2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse pancreas using 14157-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using KRAP/SSFA2 antibody (14157-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse pancreas using 14157-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using KRAP/SSFA2 antibody (14157-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse lung tissue, mouse testis tissue, rat lung tissue
• Positive IP detected in: mouse testis tissue
• Positive IHC detected in: human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse pancreas tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 7 publications below
• IHC: See 2 publications below
• IF: See 3 publications below
• CoIP: See 1 publications below

Product Information

14157-1-AP targets KRAP/SSFA2 in WB, IHC, IF-P, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, canine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: KRAP/SSFA2 fusion protein Ag5339
• Full Name: sperm specific antigen 2
• Calculated Molecular Weight: 138 kDa
• Observed Molecular Weight: 180 kDa
• GenBank Accession Number: BC028706
• Gene Symbol: SSFA2
• Gene ID (NCBI): 6744
• RRID: AB_2195472
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KRAP (Ki-ras-induced actin-interacting protein), also known as SSFA2, CS1 or SPAG13, is a 1,259 amino acid cytoplasmic protein associated with filamentous-actin (F-actin). KRAP is strongly expressed in pancreas and testis. It is also present in colon cancer cell lines, and the expression is up-regulated by activated K-Ras. KRAP-deficient (KO) mice display decreased adiposity and improved glucose metabolism, implicating KRAP as a potential target for the prevention or treatment of obesity and related diseases. KRAP has also been suggested to be a potential target for cancer. This antibody recognizes endogenous KRAP, which migrates with an apparent molecular mass of 180 kDa in SDS-PAGE. (PMID: 14673706; 21501587; 21873152)

Publications

Species	Application	Title
human	WB,IHC	Nat Commun; KRAP tethers IP3 receptors to actin and licenses them to evoke cytosolic Ca2+ signals.; Authors - Nagendra Babu Thillaiappan ; KD Validated
canine	WB, IF	J Cell Sci; Vimentin and the K-Ras-induced actin-binding protein control inositol-(1,4,5)-trisphosphate receptor redistribution during MDCK cell differentiation.; Authors - Dingli Florent F ; KD Validated
human	WB,IHC	J Proteomics; Relationship between phosphorylation of sperm-specific antigen and prognosis of lung adenocarcinoma.; Authors - Akiko Okayama
human	WB,IF,CoIP	Reprod Biol Endocrinol; A loss-of-function variant in SSFA2 causes male infertility with globozoospermia and failed oocyte activation.; Authors - Gelin Huang
human	WB	Front Immunol; Identification of a novel cuproptosis-related gene signature and integrative analyses in patients with lower-grade gliomas; Authors - Jia-hao Bao
human	WB	J Cell Sci; CREB regulates the expression of type 1 inositol 1,4,5-trisphosphate receptors; Authors - Vikas Arige